Mitochondrial alterations of skeletal muscle in a heat stress rat model.

Y. D. Hsu, S. S. Chen, W. H. Lee, S. Z. Lin, M. C. Kao, W. L. Tsao

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)


The morphological and ultrastructural alterations of skeletal muscle in experimental rats with heat stress were investigated. Fifteen male Sprague-Dawley rats were exposed in a 42 degrees C constant temperature oven, resulting in a heat stress state; ten rats were used as controls. All treated rats had weakness of the 4 limbs associated with increased serum creatine kinase levels (p < 0.01). Soleus muscles were submitted to histological, histochemical, ultrastructural and quantitative-morphometric analysis. The group receiving heat stress showed many ragged-red fibers in Gomori trichrome stain and appeared hyper-reactive in succinate dehydrogenase and cytochrome C oxidase stains. The ultrastructure of ragged-red fibers showed increased mitochondrial aggregation as multiple small nests, which were particularly located in the subsarcolemmal space. The mitochondrial area was significantly increased in heat stress rats (p < 0.001). The consistently increased mitochondrial area and histochemical alterations of mitochondria are early pathological abnormalities in muscles with heat stress and indicate fundamental impairment of energy metabolism.

Original languageEnglish
Pages (from-to)233-239
Number of pages7
JournalProceedings of the National Science Council, Republic of China. Part B, Life sciences
Issue number4
Publication statusPublished - Oct 1995
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)


Dive into the research topics of 'Mitochondrial alterations of skeletal muscle in a heat stress rat model.'. Together they form a unique fingerprint.

Cite this