miR-145 mediates the anti-cancer stemness effect of photodynamic therapy with 5-aminolevulinic acid (ALA) in oral cancer cells

Chih Yuan Fang; Pei Yin Chen; Dennis Chun Yu Ho; Lo Lin Tsai; Pei Ling Hsieh; Ming Yi Lu; Cheng Chia Yu; Chuan Hang Yu

doi:10.1016/j.jfma.2018.05.018

miR-145 mediates the anti-cancer stemness effect of photodynamic therapy with 5-aminolevulinic acid (ALA) in oral cancer cells

Chih Yuan Fang, Pei Yin Chen, Dennis Chun Yu Ho, Lo Lin Tsai, Pei Ling Hsieh, Ming Yi Lu, Cheng Chia Yu, Chuan Hang Yu

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

5-Aminolevulinic acid-mediated photodynamic therapy (ALA-PDT) has been used in the treatment of various precancerous and malignant lesions. Our previous work has demonstrated that ALA-PDT possesses the potential to serve as an adjuvant therapy against head and neck cancer via eliminating the cancer stem cells (CSCs) property. This study aimed to further investigate the possible molecular mechanism underlying the effect of ALA-PDT. Our results revealed that ALA-PDT upregulated the expression of microRNA-145 (miR-145) in two oral cancer cell lines. Overexpression of miR-145 in oral CSCs further enhanced the treatment effect of ALA-PDT with lower self-renewal, invasion capacities and reduced CD44 expression, while inhibition of miR-145 exhibited the opposite phenomena. These findings suggest that the anti-CSCs effect of ALA-PDT is due to an elevation of miR-145.

Original language	English
Pages (from-to)	738-742
Number of pages	5
Journal	Journal of the Formosan Medical Association
Volume	117
Issue number	8
DOIs	https://doi.org/10.1016/j.jfma.2018.05.018
Publication status	Published - Aug 1 2018

Keywords

5-Aminolevulinic acid-mediated photodynamic therapy
microRNA-145
Oral cancer stem cells

ASJC Scopus subject areas

Medicine(all)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.jfma.2018.05.018

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@article{1ac91e6a8a2e4af69b03d628f0eb0fe8,

title = "miR-145 mediates the anti-cancer stemness effect of photodynamic therapy with 5-aminolevulinic acid (ALA) in oral cancer cells",

abstract = "5-Aminolevulinic acid-mediated photodynamic therapy (ALA-PDT) has been used in the treatment of various precancerous and malignant lesions. Our previous work has demonstrated that ALA-PDT possesses the potential to serve as an adjuvant therapy against head and neck cancer via eliminating the cancer stem cells (CSCs) property. This study aimed to further investigate the possible molecular mechanism underlying the effect of ALA-PDT. Our results revealed that ALA-PDT upregulated the expression of microRNA-145 (miR-145) in two oral cancer cell lines. Overexpression of miR-145 in oral CSCs further enhanced the treatment effect of ALA-PDT with lower self-renewal, invasion capacities and reduced CD44 expression, while inhibition of miR-145 exhibited the opposite phenomena. These findings suggest that the anti-CSCs effect of ALA-PDT is due to an elevation of miR-145.",

keywords = "5-Aminolevulinic acid-mediated photodynamic therapy, microRNA-145, Oral cancer stem cells",

author = "Fang, {Chih Yuan} and Chen, {Pei Yin} and Ho, {Dennis Chun Yu} and Tsai, {Lo Lin} and Hsieh, {Pei Ling} and Lu, {Ming Yi} and Yu, {Cheng Chia} and Yu, {Chuan Hang}",

note = "Funding Information: This study was supported by grants from Wan Fang Hospital ( 107-wf-eva-03 ) in Taiwan. Publisher Copyright: {\textcopyright} 2018",

year = "2018",

month = aug,

day = "1",

doi = "10.1016/j.jfma.2018.05.018",

language = "English",

volume = "117",

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journal = "Journal of the Formosan Medical Association",

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TY - JOUR

T1 - miR-145 mediates the anti-cancer stemness effect of photodynamic therapy with 5-aminolevulinic acid (ALA) in oral cancer cells

AU - Fang, Chih Yuan

AU - Chen, Pei Yin

AU - Ho, Dennis Chun Yu

AU - Tsai, Lo Lin

AU - Hsieh, Pei Ling

AU - Lu, Ming Yi

AU - Yu, Cheng Chia

AU - Yu, Chuan Hang

PY - 2018/8/1

Y1 - 2018/8/1

N2 - 5-Aminolevulinic acid-mediated photodynamic therapy (ALA-PDT) has been used in the treatment of various precancerous and malignant lesions. Our previous work has demonstrated that ALA-PDT possesses the potential to serve as an adjuvant therapy against head and neck cancer via eliminating the cancer stem cells (CSCs) property. This study aimed to further investigate the possible molecular mechanism underlying the effect of ALA-PDT. Our results revealed that ALA-PDT upregulated the expression of microRNA-145 (miR-145) in two oral cancer cell lines. Overexpression of miR-145 in oral CSCs further enhanced the treatment effect of ALA-PDT with lower self-renewal, invasion capacities and reduced CD44 expression, while inhibition of miR-145 exhibited the opposite phenomena. These findings suggest that the anti-CSCs effect of ALA-PDT is due to an elevation of miR-145.

AB - 5-Aminolevulinic acid-mediated photodynamic therapy (ALA-PDT) has been used in the treatment of various precancerous and malignant lesions. Our previous work has demonstrated that ALA-PDT possesses the potential to serve as an adjuvant therapy against head and neck cancer via eliminating the cancer stem cells (CSCs) property. This study aimed to further investigate the possible molecular mechanism underlying the effect of ALA-PDT. Our results revealed that ALA-PDT upregulated the expression of microRNA-145 (miR-145) in two oral cancer cell lines. Overexpression of miR-145 in oral CSCs further enhanced the treatment effect of ALA-PDT with lower self-renewal, invasion capacities and reduced CD44 expression, while inhibition of miR-145 exhibited the opposite phenomena. These findings suggest that the anti-CSCs effect of ALA-PDT is due to an elevation of miR-145.

KW - 5-Aminolevulinic acid-mediated photodynamic therapy

KW - microRNA-145

KW - Oral cancer stem cells

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miR-145 mediates the anti-cancer stemness effect of photodynamic therapy with 5-aminolevulinic acid (ALA) in oral cancer cells

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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