TY - JOUR
T1 - MicroRNA 363 mediated positive regulation of c-myc translation affect prostate cancer development and progress
AU - Chen, Y.
AU - Lu, X.
AU - Wu, B.
AU - Su, Y.
AU - Li, J.
AU - Wang, H.
N1 - Publisher Copyright:
© 2015, Cancer Research Institute Slovak Acad. of Sciences. All rights reserved.
PY - 2015
Y1 - 2015
N2 - Prostate cancer (CaP) is the sixth most significant cancer killer of men in China. In this study, the potential role of micro-363 (miR-363) in CaP development and progression was investigated. Pri-miR-363 or anti-miR-363 was transfected into the CaP cells line PC-3 cells. Cell proliferation, transformation property, and epithelial-to-mesenchymal transition (EMT) were evaluated by MTT, clonogenic assay, colony formation in soft agar and western blotting, respectively. The expression and involvement of c-myc, a downstream target of miR-363 were also determined. The results showed that endogenous expression of miR-363 was significantly increased in CaP cells compared with normal prostate cells. High expression of miR-363 in PC-3 cells through transfection induces cell proliferation and positively regulates cell transformation property as well as promotes EMT of PC-3 cells. Through knockdown of c-myc, the results also showed that c-myc was involved in the regulation of biological function of PC-3 cells by miR-363. Taken together, this study adds support to the potential role of miR-363 in the diagnosis and treatment of CaP.
AB - Prostate cancer (CaP) is the sixth most significant cancer killer of men in China. In this study, the potential role of micro-363 (miR-363) in CaP development and progression was investigated. Pri-miR-363 or anti-miR-363 was transfected into the CaP cells line PC-3 cells. Cell proliferation, transformation property, and epithelial-to-mesenchymal transition (EMT) were evaluated by MTT, clonogenic assay, colony formation in soft agar and western blotting, respectively. The expression and involvement of c-myc, a downstream target of miR-363 were also determined. The results showed that endogenous expression of miR-363 was significantly increased in CaP cells compared with normal prostate cells. High expression of miR-363 in PC-3 cells through transfection induces cell proliferation and positively regulates cell transformation property as well as promotes EMT of PC-3 cells. Through knockdown of c-myc, the results also showed that c-myc was involved in the regulation of biological function of PC-3 cells by miR-363. Taken together, this study adds support to the potential role of miR-363 in the diagnosis and treatment of CaP.
KW - Epithelial-to-mesenchymal transition
KW - Micro-363
KW - Proliferation
KW - Prostate cancer
KW - Transformation property
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UR - http://www.scopus.com/inward/citedby.url?scp=84943601257&partnerID=8YFLogxK
U2 - 10.4149/neo_2015_024
DO - 10.4149/neo_2015_024
M3 - Article
AN - SCOPUS:84943601257
SN - 0028-2685
VL - 62
SP - 191
EP - 198
JO - Neoplasma
JF - Neoplasma
IS - 2
ER -
