Microglia-derived glial cell line-derived neurotrophic factor could protect Sprague-Dawley rat astrocyte from in vitro ischemia-induced damage

Gilbert A. Lee; Chi Hsin Lin; Hang Hsiang Jiang; Hsin Ju Chao; Chieh Liang Wu; Chi Mei Hsueh

doi:10.1016/j.neulet.2003.11.030

Microglia-derived glial cell line-derived neurotrophic factor could protect Sprague-Dawley rat astrocyte from in vitro ischemia-induced damage

Gilbert A. Lee, Chi Hsin Lin, Hang Hsiang Jiang, Hsin Ju Chao, Chieh Liang Wu, Chi Mei Hsueh

Research output: Contribution to journal › Article › peer-review

28 Citations (Scopus)

Abstract

The interrelationship between microglia and astrocytes in cerebral ischemia was determined in vitro by adding in vitro ischemia-induced supernatant from microglia into astrocytes under the same conditions (glucose-, oxygen- and serum-free). The involvement of glial cell line-derived neurotrophic factor (GDNF) was further investigated by immunoblocking assay and Western blot analysis. Results showed that microglia-derived supernatant protected against in vitro ischemia-induced damage of astrocytes and this protection was pre-blocked by anti-GDNF but not normal rabbit serum. In addition, in vitro ischemia appeared to induce the expression of GDNF in microglia. These results indicate that microglia-derived protection on astrocytes during in vitro ischemia is GDNF-dependent.

Original language	English
Pages (from-to)	111-114
Number of pages	4
Journal	Neuroscience Letters
Volume	356
Issue number	2
DOIs	https://doi.org/10.1016/j.neulet.2003.11.030
Publication status	Published - Feb 12 2004

Keywords

Astrocyte
Glial cell line-derived neurotrophic factor
In vitro ischemia
Microglia

ASJC Scopus subject areas

General Neuroscience

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10.1016/j.neulet.2003.11.030

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title = "Microglia-derived glial cell line-derived neurotrophic factor could protect Sprague-Dawley rat astrocyte from in vitro ischemia-induced damage",

abstract = "The interrelationship between microglia and astrocytes in cerebral ischemia was determined in vitro by adding in vitro ischemia-induced supernatant from microglia into astrocytes under the same conditions (glucose-, oxygen- and serum-free). The involvement of glial cell line-derived neurotrophic factor (GDNF) was further investigated by immunoblocking assay and Western blot analysis. Results showed that microglia-derived supernatant protected against in vitro ischemia-induced damage of astrocytes and this protection was pre-blocked by anti-GDNF but not normal rabbit serum. In addition, in vitro ischemia appeared to induce the expression of GDNF in microglia. These results indicate that microglia-derived protection on astrocytes during in vitro ischemia is GDNF-dependent.",

keywords = "Astrocyte, Glial cell line-derived neurotrophic factor, In vitro ischemia, Microglia",

author = "Lee, \{Gilbert A.\} and Lin, \{Chi Hsin\} and Jiang, \{Hang Hsiang\} and Chao, \{Hsin Ju\} and Wu, \{Chieh Liang\} and Hsueh, \{Chi Mei\}",

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T1 - Microglia-derived glial cell line-derived neurotrophic factor could protect Sprague-Dawley rat astrocyte from in vitro ischemia-induced damage

AU - Lee, Gilbert A.

AU - Lin, Chi Hsin

AU - Jiang, Hang Hsiang

AU - Chao, Hsin Ju

AU - Wu, Chieh Liang

AU - Hsueh, Chi Mei

PY - 2004/2/12

Y1 - 2004/2/12

N2 - The interrelationship between microglia and astrocytes in cerebral ischemia was determined in vitro by adding in vitro ischemia-induced supernatant from microglia into astrocytes under the same conditions (glucose-, oxygen- and serum-free). The involvement of glial cell line-derived neurotrophic factor (GDNF) was further investigated by immunoblocking assay and Western blot analysis. Results showed that microglia-derived supernatant protected against in vitro ischemia-induced damage of astrocytes and this protection was pre-blocked by anti-GDNF but not normal rabbit serum. In addition, in vitro ischemia appeared to induce the expression of GDNF in microglia. These results indicate that microglia-derived protection on astrocytes during in vitro ischemia is GDNF-dependent.

AB - The interrelationship between microglia and astrocytes in cerebral ischemia was determined in vitro by adding in vitro ischemia-induced supernatant from microglia into astrocytes under the same conditions (glucose-, oxygen- and serum-free). The involvement of glial cell line-derived neurotrophic factor (GDNF) was further investigated by immunoblocking assay and Western blot analysis. Results showed that microglia-derived supernatant protected against in vitro ischemia-induced damage of astrocytes and this protection was pre-blocked by anti-GDNF but not normal rabbit serum. In addition, in vitro ischemia appeared to induce the expression of GDNF in microglia. These results indicate that microglia-derived protection on astrocytes during in vitro ischemia is GDNF-dependent.

KW - Astrocyte

KW - Glial cell line-derived neurotrophic factor

KW - In vitro ischemia

KW - Microglia

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Microglia-derived glial cell line-derived neurotrophic factor could protect Sprague-Dawley rat astrocyte from in vitro ischemia-induced damage

Abstract

Keywords

ASJC Scopus subject areas

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