Abstract
The methanol-soluble sugar hydroxamic acid derivatives obtained during the preparation of alginic acid hydroxamates (AAH) were separated by BioSil-ODS HPLC column (10x250 mm) in acetonitrite: 0.05% trifluoroacetic acid, 10:90 (V/V). The absorbance at 235 nm was set for monitoring β-elimination products. Each fraction was collected and assayed for hydroxamic acid contents, 1, 1-diphenyl-2-picrylhydrazyl (DPPH) scavenging activity, and inhibitory activity against angiotensin converting enzyme (ACE). The fraction with higher hydroxamic acid content was further separated by Sephadex G-15 column. Fractions containing both ACE inhibitory activity and DPPH scavenging activity overlapped with fractions containing high levels of hydroxamic acid derivatives. The chromatogram showed little tailing when judged by hydroxamic-acid content but significant tailing when judged by biological activity. Each fraction from above was further separated by silica-TLC in acetonitrite: distilled water, 95:5 (V/V). It was found that a methanol soluble fraction prepared from alginic acid hydroxamates (AAH) exhibited DPPH scavenging activity.
| Original language | English |
|---|---|
| Pages (from-to) | 141-146 |
| Number of pages | 6 |
| Journal | Botanical Studies |
| Volume | 48 |
| Issue number | 2 |
| Publication status | Published - Apr 2007 |
Keywords
- Alginic acid hydroxamates
- Angiotensin converting enzyme (ACE)
- DPPH
- β-elimination
ASJC Scopus subject areas
- Plant Science