TY - JOUR
T1 - Metformin-Incorporated Gelatin/Hydroxyapatite Nanofiber Scaffold for Bone Regeneration
AU - Sun, Chung Kai
AU - Weng, Pei Wei
AU - Chang, Jenny Zwei Chieng
AU - Lin, Yi Wen
AU - Tsuang, Fon Yih
AU - Lin, Feng Huei
AU - Tsai, Tung Hu
AU - Sun, Jui Sheng
N1 - Funding Information:
This work was supported by the Institute of Biomedical Engineering, National Taiwan University, Taipei, Taiwan, ROC, Ministry of Science and Technology, Taipei, Taiwan, ROC [MOST 108-2314-B-002-120-MY3], and the National Taiwan University Hospital, Taipei, Taiwan, ROC [UN108-002].
Funding Information:
The authors would like to express their immense gratitude to the Institute of Biomedical Engineering, National Taiwan University, Ministry of Science and Technology, and the National Taiwan University Hospital for their financial support. We thank the staff of the Second Core Lab, Department of Medical Research, National Taiwan University Hospital, for technical support during the study. We also thank the Wizdom Inc. (Las Vegas, NV) and Ingrowth Biotech (Kaohsiung, Taiwan) for their kindness of providing the prescription or recipe of their product.
Publisher Copyright:
© Copyright 2022, Mary Ann Liebert, Inc., publishers 2022.
PY - 2022/1
Y1 - 2022/1
N2 - Tissue engineering and regenerative medicine has gradually evolved as a promising therapeutic strategy to the modern health care of aging and diseased population. In this study, we developed a novel nanofibrous scaffold and verified its application in the critical bone defect regeneration. The metformin-incorporated nano-gelatin/hydroxyapatite fibers (NGF) was produced by electrospinning, cross-linked, and then characterized by X-ray powder diffractometer and Fourier-transform infrared spectroscopy. Cytotoxicity, cell adhesion, cell differentiation, and quantitative osteogenic gene and protein expression were analyzed by bone marrow stem cells (BMSCs) from rat. Rat forearm critical bone defect model was performed for the in vivo study. The NGF were characterized by their porous structures with proper interconnectivity without significant cytotoxic effects; the adhesion of BMSCs on the NGF could be enhanced. The osteogenic gene and protein expression were upregulated. Postimplantation, the new regenerated bone in bone defect was well demonstrated in the NGF samples. We demonstrated that the metformin-incorporated NGF greatly improved healing potential on the critical-size bone defect. Although metformin-incorporated NGF had advantageous effectiveness during bone regeneration, further validation is required before it can be applied to clinical applications. Bone is the structure that supports the rest of the human body. Critical-size bone defect hinders the regeneration of damaged bone tissues and compromises the mechanical strength of the skeletal system. Characterized by their porous structures with proper interconnectivity, the electrospinning nano-gelatin/hydroxyapatite fibrous scaffold developed in this study can greatly improve the healing potential on the critical-size bone defect. Further validation can validate its potential clinical applications.
AB - Tissue engineering and regenerative medicine has gradually evolved as a promising therapeutic strategy to the modern health care of aging and diseased population. In this study, we developed a novel nanofibrous scaffold and verified its application in the critical bone defect regeneration. The metformin-incorporated nano-gelatin/hydroxyapatite fibers (NGF) was produced by electrospinning, cross-linked, and then characterized by X-ray powder diffractometer and Fourier-transform infrared spectroscopy. Cytotoxicity, cell adhesion, cell differentiation, and quantitative osteogenic gene and protein expression were analyzed by bone marrow stem cells (BMSCs) from rat. Rat forearm critical bone defect model was performed for the in vivo study. The NGF were characterized by their porous structures with proper interconnectivity without significant cytotoxic effects; the adhesion of BMSCs on the NGF could be enhanced. The osteogenic gene and protein expression were upregulated. Postimplantation, the new regenerated bone in bone defect was well demonstrated in the NGF samples. We demonstrated that the metformin-incorporated NGF greatly improved healing potential on the critical-size bone defect. Although metformin-incorporated NGF had advantageous effectiveness during bone regeneration, further validation is required before it can be applied to clinical applications. Bone is the structure that supports the rest of the human body. Critical-size bone defect hinders the regeneration of damaged bone tissues and compromises the mechanical strength of the skeletal system. Characterized by their porous structures with proper interconnectivity, the electrospinning nano-gelatin/hydroxyapatite fibrous scaffold developed in this study can greatly improve the healing potential on the critical-size bone defect. Further validation can validate its potential clinical applications.
KW - bone tissue regeneration
KW - critical-size bone defect
KW - metformin
KW - nano-gelatin/hydroxyapatite fiber
UR - http://www.scopus.com/inward/record.url?scp=85123272414&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85123272414&partnerID=8YFLogxK
U2 - 10.1089/ten.tea.2021.0038
DO - 10.1089/ten.tea.2021.0038
M3 - Article
C2 - 33971745
AN - SCOPUS:85123272414
SN - 1937-3341
VL - 28
SP - 1
EP - 12
JO - Tissue Engineering - Part A
JF - Tissue Engineering - Part A
IS - 1-2
ER -
