Metabolomics of ginger essential oil against alcoholic fatty liver in mice

Chun Ting Liu, Rajasekaran Raghu, Shu Hsi Lin, San Yuan Wang, Ching Hua Kuo, Yufeng J. Tseng, Lee Yan Sheen

Research output: Contribution to journalArticlepeer-review

45 Citations (Scopus)


Fatty liver is significantly associated with hepatic cirrhosis and liver cancer. Excessive alcohol consumption causes alcoholic fatty liver disease (AFLD). Ginger has been reported to exhibit antioxidant potential and hepatoprotective activity. In the present study, a mouse model for AFLD was developed by employing male C57BL/6 mice that were fed an alcohol-containing liquid diet (Lieber-DeCarli diet) ad libitum. In the treatment groups, ginger essential oil (GEO) and citral were orally administered every day for 4 weeks. Serum biochemical analysis, antioxidant enzyme activity analysis, and histopathological evaluation revealed that GEO and citral exhibited hepatoprotective activity against AFLD. Metabolites in serum samples were profiled by high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (HPLC-QTOF-MS). Metabolomic data indicated the amounts of metabolites such as d-glucurono-6,3-lactone, glycerol-3-phosphate, pyruvic acid, lithocholic acid, 2-pyrocatechuic acid, and prostaglandin E1 were increased after alcohol administration, but the levels were recovered in treatment groups. The analysis indicated that ginger possesses hepatoprotective properties against AFLD. Furthermore, these metabolites can serve as early noninvasive candidate biomarkers in the clinical application of AFLD for health management.

Original languageEnglish
Pages (from-to)11231-11240
Number of pages10
JournalJournal of Agricultural and Food Chemistry
Issue number46
Publication statusPublished - Nov 20 2013
Externally publishedYes


  • AFLD
  • biomarker
  • ginger
  • Lieber-DeCarli diet
  • metabolomics

ASJC Scopus subject areas

  • General Chemistry
  • General Agricultural and Biological Sciences


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