TY - JOUR
T1 - Metabolic detriment in donor heart valves induced by ischemia and cryopreservation
AU - Lu, Jen Her
AU - Chang, Yen
AU - Hsu, Wen Hu
AU - Hwang, Betau
AU - Chong, Chuhkhiun
AU - Wu, Chi Chin
AU - Yang, Ping Zen
AU - Hsing-Wen, Hsung
N1 - Funding Information:
This research was supported by the National Scientific Council (NSC 83-0412-B-075-063), Taiwan, ROC.
Copyright:
Copyright 2007 Elsevier B.V., All rights reserved.
PY - 1998/1
Y1 - 1998/1
N2 - Background. The injury resulting from postmortem ischemia is a critical deterrent to the availability of donor valves. Using the reduction of XTT- tetrazolium salt as a marker of metabolic sequelae, we assessed the injurious effect of ischemia and the metabolic sequelae in 156 porcine semilunar leaflets. Methods. The leaflets were randomly allocated to noncryoprocessed (n = 72) or cryoprocessed (n = 72) groups. At each preservation temperature of 4°C, 24 °C, or 37°C, 24 leaflets each were exposed to one of four storage periods of 9, 17, 30 or 60 hours. Twelve fresh aortic leaflets served as baseline reference samples. Results. There was a progressive loss in the metabolic functioning of valve leaflet cells in both noncryopreserved and cryopreserved tissue as the storage times increased. Cryopreserved tissue showed a greater loss of function than noncryopreserved tissue did. The metabolic injury was mainly a consequence of cryoprocessing. The greatest loss in metabolic functioning occurred in the valves stored for 60 hours. The least favorable combination of variables was cryopreservation and a precryopreservation storage time of 60 hours. Conclusions. We conclude that 30- to 60-hour delays do not have a significant metabolic effect on cardiac leaflets. Thus it may be possible to safely extend the permissible ischemic periods after organ harvest.
AB - Background. The injury resulting from postmortem ischemia is a critical deterrent to the availability of donor valves. Using the reduction of XTT- tetrazolium salt as a marker of metabolic sequelae, we assessed the injurious effect of ischemia and the metabolic sequelae in 156 porcine semilunar leaflets. Methods. The leaflets were randomly allocated to noncryoprocessed (n = 72) or cryoprocessed (n = 72) groups. At each preservation temperature of 4°C, 24 °C, or 37°C, 24 leaflets each were exposed to one of four storage periods of 9, 17, 30 or 60 hours. Twelve fresh aortic leaflets served as baseline reference samples. Results. There was a progressive loss in the metabolic functioning of valve leaflet cells in both noncryopreserved and cryopreserved tissue as the storage times increased. Cryopreserved tissue showed a greater loss of function than noncryopreserved tissue did. The metabolic injury was mainly a consequence of cryoprocessing. The greatest loss in metabolic functioning occurred in the valves stored for 60 hours. The least favorable combination of variables was cryopreservation and a precryopreservation storage time of 60 hours. Conclusions. We conclude that 30- to 60-hour delays do not have a significant metabolic effect on cardiac leaflets. Thus it may be possible to safely extend the permissible ischemic periods after organ harvest.
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U2 - 10.1016/S0003-4975(97)00913-2
DO - 10.1016/S0003-4975(97)00913-2
M3 - Article
C2 - 9456089
AN - SCOPUS:0031930015
SN - 0003-4975
VL - 65
SP - 24
EP - 27
JO - Annals of Thoracic Surgery
JF - Annals of Thoracic Surgery
IS - 1
ER -