Mesoporous silica nanoparticles improve magnetic labeling efficiency in human stem cells

Hon Man Liu, Si Han Wu, Chen Wen Lu, Ming Yao, Jong Kai Hsiao, Yann Hung, Yu Shen Lin, Chung Yuan Mou, Chung Shi Yang, Dong Ming Huang, Yao Chang Chen

Research output: Contribution to journalArticlepeer-review

124 Citations (Scopus)


Tumblerlike magnetic/fluorescein isothiocyanate (FITC)-labeled mesoporous silica nanoparticles, Mag-Dye@MSNs, have been developed, which are composed of silica-coated core-shell superparamagnetic iron oxide (SPICK&SiO 2) nanoparticles co-condensed with FITC-incorporated mesoporous silica. Mag-Dye@MSNs can label human mesenchymal stem cells (hMSCs) through endocytosis efficiently for magnetic resonance imaging (MRI) in vitro and in vivo, as manifested by using a clinical 1.5-T MRI system with requirements of simultaneous low incubation dosage of iron, low detection cell numbers, and short incubation time. Labeled hMSCs are unaffected in their viability, proliferation, and differentiation capacities into adipocytes and osteocytes, which can still be readily detected by MRI. Moreover, a higher MRI signal intensity decrease is observed in Mag-Dye@MSN-treated cells than in SPIO@SiO2-treated cells. This is the first report that MCM-41-type MSNs are advantageous to cellular uptake, as manifested by a higher labeling efficiency of Mag-Dye@MSNs than SPIO@SiO2.

Original languageEnglish
Pages (from-to)619-626
Number of pages8
Issue number5
Publication statusPublished - May 2008
Externally publishedYes


  • Biocompatibte materials
  • Magnetic resonance imaging
  • Mesoporous materials
  • Nanoparticles
  • Stem cells

ASJC Scopus subject areas

  • Chemistry(all)
  • Materials Science(all)
  • Biotechnology
  • Biomaterials


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