Mesenchymal stem cell attachment to peptide density gradients on porous silicon generated by electrografting

Lauren R. Clements; Peng Yuan Wang; Frances Harding; Wei Bor Tsai; Helmut Thissen; Nicolas H. Voelcker

doi:10.1002/pssa.201000320

Mesenchymal stem cell attachment to peptide density gradients on porous silicon generated by electrografting

Lauren R. Clements, Peng Yuan Wang, Frances Harding, Wei Bor Tsai, Helmut Thissen, Nicolas H. Voelcker

Research output: Contribution to journal › Article › peer-review

31 Citations (Scopus)

Abstract

Chemical gradients of ethyl-6-bromohexanoate (EBH) were generated over porous silicon (pSi) substrates via electrochemical attachment. Immobilised ester moieties were hydrolysed and activated to produce a gradient of functional carboxylic acid groups. After subsequent immobilisation of cyclic RGD (cRGD), the surfaces were used to screen the extent of rat mesenchymal stem cell (MSC) attachment. Mapping of surface chemistry was carried out by means of infrared microscopy and X-ray photoelectron spectroscopy (XPS). MSC culture studies showed that short-term cell attachment responded to the cRGD density present on the gradient surface.

Original language	English
Pages (from-to)	1440-1445
Number of pages	6
Journal	Physica Status Solidi (A) Applications and Materials Science
Volume	208
Issue number	6
DOIs	https://doi.org/10.1002/pssa.201000320
Publication status	Published - Jun 2011
Externally published	Yes

Keywords

biomaterial
electrochemistry
gradient surfaces
porous silicon
surface chemistry

ASJC Scopus subject areas

Electrical and Electronic Engineering
Electronic, Optical and Magnetic Materials
Materials Chemistry
Condensed Matter Physics
Surfaces and Interfaces
Surfaces, Coatings and Films

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10.1002/pssa.201000320

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abstract = "Chemical gradients of ethyl-6-bromohexanoate (EBH) were generated over porous silicon (pSi) substrates via electrochemical attachment. Immobilised ester moieties were hydrolysed and activated to produce a gradient of functional carboxylic acid groups. After subsequent immobilisation of cyclic RGD (cRGD), the surfaces were used to screen the extent of rat mesenchymal stem cell (MSC) attachment. Mapping of surface chemistry was carried out by means of infrared microscopy and X-ray photoelectron spectroscopy (XPS). MSC culture studies showed that short-term cell attachment responded to the cRGD density present on the gradient surface.",

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T1 - Mesenchymal stem cell attachment to peptide density gradients on porous silicon generated by electrografting

AU - Clements, Lauren R.

AU - Wang, Peng Yuan

AU - Harding, Frances

AU - Tsai, Wei Bor

AU - Thissen, Helmut

AU - Voelcker, Nicolas H.

PY - 2011/6

Y1 - 2011/6

N2 - Chemical gradients of ethyl-6-bromohexanoate (EBH) were generated over porous silicon (pSi) substrates via electrochemical attachment. Immobilised ester moieties were hydrolysed and activated to produce a gradient of functional carboxylic acid groups. After subsequent immobilisation of cyclic RGD (cRGD), the surfaces were used to screen the extent of rat mesenchymal stem cell (MSC) attachment. Mapping of surface chemistry was carried out by means of infrared microscopy and X-ray photoelectron spectroscopy (XPS). MSC culture studies showed that short-term cell attachment responded to the cRGD density present on the gradient surface.

AB - Chemical gradients of ethyl-6-bromohexanoate (EBH) were generated over porous silicon (pSi) substrates via electrochemical attachment. Immobilised ester moieties were hydrolysed and activated to produce a gradient of functional carboxylic acid groups. After subsequent immobilisation of cyclic RGD (cRGD), the surfaces were used to screen the extent of rat mesenchymal stem cell (MSC) attachment. Mapping of surface chemistry was carried out by means of infrared microscopy and X-ray photoelectron spectroscopy (XPS). MSC culture studies showed that short-term cell attachment responded to the cRGD density present on the gradient surface.

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KW - electrochemistry

KW - gradient surfaces

KW - porous silicon

KW - surface chemistry

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JO - Physica Status Solidi (A) Applications and Materials Science

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Mesenchymal stem cell attachment to peptide density gradients on porous silicon generated by electrografting

Abstract

Keywords

ASJC Scopus subject areas

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