Mechanistic aspects of CoII(HAPP)(TFA)2 in DNA bulge-specific recognition

Chien Chung Cheng; Wei Chun Huang-Fu; Kuo Chan Hung; Po Jui Chen; Wen Jwu Wang; Yu Ju Chen

doi:10.1093/nar/gkg323

Mechanistic aspects of Co^II(HAPP)(TFA)₂ in DNA bulge-specific recognition

Chien Chung Cheng, Wei Chun Huang-Fu, Kuo Chan Hung, Po Jui Chen, Wen Jwu Wang, Yu Ju Chen

Research output: Contribution to journal › Article › peer-review

34 Citations (Scopus)

Abstract

A novel octahedral complex Co^II(HAPP)(TFA)₂ [hexa-azaphenantholine-cyclophane (HAPP), trifluoroacetate (TFA)] is a DNA bulge-specific probe with single-strand DNA cleavage activity in the presence of H₂O₂. This complex exhibits low affinity towards double-stranded DNA and low reactivity toward single-stranded DNA. Metal-HAPP complexes with different coordination number and ring size were synthesized and their selectivity and reactivity for DNA bulges were compared. The DNA sequence at the bulge site influences the intensity of cleavage at the bulge and the flanking sites after piperidine treatment. Cleavage specificity of Co^II(HAPP)(TFA)₂ was characterized extensively using scavenger reagents to quench the cleavage reaction and high-resolution polyacrylamide gel electrophoresis. In addition, 3′-phosphoglycolate cleavage products were trapped and analyzed by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. These data were used to deduce that the DNA cleavage pathway for Co^IIHAPP²⁺ in the presence of H₂O₂ involves 4′-H abstraction of the deoxyribose moiety.

Original language	English
Pages (from-to)	2227-2233
Number of pages	7
Journal	Nucleic Acids Research
Volume	31
Issue number	8
DOIs	https://doi.org/10.1093/nar/gkg323
Publication status	Published - Apr 15 2003
Externally published	Yes

ASJC Scopus subject areas

Genetics

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@article{e7350ff66faf469eb7fcb65d89d776be,

title = "Mechanistic aspects of CoII(HAPP)(TFA)2 in DNA bulge-specific recognition",

abstract = "A novel octahedral complex CoII(HAPP)(TFA)2 [hexa-azaphenantholine-cyclophane (HAPP), trifluoroacetate (TFA)] is a DNA bulge-specific probe with single-strand DNA cleavage activity in the presence of H2O2. This complex exhibits low affinity towards double-stranded DNA and low reactivity toward single-stranded DNA. Metal-HAPP complexes with different coordination number and ring size were synthesized and their selectivity and reactivity for DNA bulges were compared. The DNA sequence at the bulge site influences the intensity of cleavage at the bulge and the flanking sites after piperidine treatment. Cleavage specificity of CoII(HAPP)(TFA)2 was characterized extensively using scavenger reagents to quench the cleavage reaction and high-resolution polyacrylamide gel electrophoresis. In addition, 3′-phosphoglycolate cleavage products were trapped and analyzed by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. These data were used to deduce that the DNA cleavage pathway for CoIIHAPP2+ in the presence of H2O2 involves 4′-H abstraction of the deoxyribose moiety.",

author = "Cheng, {Chien Chung} and Huang-Fu, {Wei Chun} and Hung, {Kuo Chan} and Chen, {Po Jui} and Wang, {Wen Jwu} and Chen, {Yu Ju}",

note = "Funding Information: Financial support from Academia Sinica, Tamkang University and the National Science Council of Republic of China is gratefully acknowledged.",

year = "2003",

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doi = "10.1093/nar/gkg323",

language = "English",

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AU - Cheng, Chien Chung

AU - Huang-Fu, Wei Chun

AU - Hung, Kuo Chan

AU - Chen, Po Jui

AU - Wang, Wen Jwu

AU - Chen, Yu Ju

N1 - Funding Information: Financial support from Academia Sinica, Tamkang University and the National Science Council of Republic of China is gratefully acknowledged.

PY - 2003/4/15

Y1 - 2003/4/15

N2 - A novel octahedral complex CoII(HAPP)(TFA)2 [hexa-azaphenantholine-cyclophane (HAPP), trifluoroacetate (TFA)] is a DNA bulge-specific probe with single-strand DNA cleavage activity in the presence of H2O2. This complex exhibits low affinity towards double-stranded DNA and low reactivity toward single-stranded DNA. Metal-HAPP complexes with different coordination number and ring size were synthesized and their selectivity and reactivity for DNA bulges were compared. The DNA sequence at the bulge site influences the intensity of cleavage at the bulge and the flanking sites after piperidine treatment. Cleavage specificity of CoII(HAPP)(TFA)2 was characterized extensively using scavenger reagents to quench the cleavage reaction and high-resolution polyacrylamide gel electrophoresis. In addition, 3′-phosphoglycolate cleavage products were trapped and analyzed by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. These data were used to deduce that the DNA cleavage pathway for CoIIHAPP2+ in the presence of H2O2 involves 4′-H abstraction of the deoxyribose moiety.

AB - A novel octahedral complex CoII(HAPP)(TFA)2 [hexa-azaphenantholine-cyclophane (HAPP), trifluoroacetate (TFA)] is a DNA bulge-specific probe with single-strand DNA cleavage activity in the presence of H2O2. This complex exhibits low affinity towards double-stranded DNA and low reactivity toward single-stranded DNA. Metal-HAPP complexes with different coordination number and ring size were synthesized and their selectivity and reactivity for DNA bulges were compared. The DNA sequence at the bulge site influences the intensity of cleavage at the bulge and the flanking sites after piperidine treatment. Cleavage specificity of CoII(HAPP)(TFA)2 was characterized extensively using scavenger reagents to quench the cleavage reaction and high-resolution polyacrylamide gel electrophoresis. In addition, 3′-phosphoglycolate cleavage products were trapped and analyzed by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. These data were used to deduce that the DNA cleavage pathway for CoIIHAPP2+ in the presence of H2O2 involves 4′-H abstraction of the deoxyribose moiety.

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Mechanistic aspects of Co^II(HAPP)(TFA)₂ in DNA bulge-specific recognition

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