Mechanisms by which interleukin-6 attenuates cell invasion and tumorigenesis in human bladder carcinoma cells

Ke Hung Tsui, Shyi Wu Wang, Li Chuan Chung, Tsui Hsia Feng, Tzu Yi Lee, Phei Lang Chang, Horng Heng Juang

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)

Abstract

Interleukin-6, a multifunctional cytokine, contributes to tumor cell proliferation and differentiation. However, the biological mechanisms that are affected by the expression of interleukin-6 in bladder cancer cells remain unclear. We evaluated the effects of interleukin-6 expression in human bladder carcinoma cells in vitro and in vivo. The results of interleukin-6-knockdown experiments in T24 cells and interleukin-6-overexpression experiments in HT1376 cells revealed that interleukin-6 reduced cell proliferation, migration, and invasion in vitro. Xenograft animal studies indicated that the overexpression of interleukin-6 downregulated tumorigenesis of bladder cells and that interleukin-6 knockdown reversed this effect. The results of RT-PCR, immunoblotting, and reporter assays indicated that the overexpression of interleukin-6 upregulated the expression of the mammary serine protease inhibitor (MASPIN), N-myc downstream gene 1 (NDRG1), and KAI1 proteins in HT1376 cells and that interleukin-6 knockdown reduced the expression of these proteins in T24 cells. In addition, results of immunoblotting assays revealed that interleukin-6 modulated epithelial-mesenchymal transitions by upregulating the expression of the E-cadherin, while downregulation N-cadherin and vimentin proteins. Our results suggest that the effects of interleukin-6 on the regulation of epithelial-mesenchymal transitions and the expressions of the MASPIN, NDRG1, and KAI1 genes attribute to the modulation of tumorigenesis in human bladder carcinoma cells.

Original languageEnglish
Article number791212
JournalBioMed Research International
Volume2013
DOIs
Publication statusPublished - 2013
Externally publishedYes

ASJC Scopus subject areas

  • General Immunology and Microbiology
  • General Biochemistry,Genetics and Molecular Biology

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