Mechanism of Taiwan Mingjian Oolong Tea to Inhibit Isoproterenol-Induced Hypertrophy and Apoptosis in Cardiomyoblasts

Yu Lan Yeh; Hsiang I. Tsai; Shiu Min Cheng; Peiying Pai; Tsung Jung Ho; Ray Jade Chen; Chao Hung Lai; Pei Jane Huang; V. Vijaya Padma; Chih Yang Huang

doi:10.1142/S0192415X16500051

Mechanism of Taiwan Mingjian Oolong Tea to Inhibit Isoproterenol-Induced Hypertrophy and Apoptosis in Cardiomyoblasts

Yu Lan Yeh, Hsiang I. Tsai, Shiu Min Cheng, Peiying Pai, Tsung Jung Ho, Ray Jade Chen, Chao Hung Lai, Pei Jane Huang, V. Vijaya Padma, Chih Yang Huang

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

This study investigates the cardio-protective effect of Nos. 1 and 5 extracts from Taiwan Mingjian Oolong Tea on H9c2 cardiomyoblast cells treated with isoproterenol (ISO). Treatment with Nos. 1 and 5 extracts increased cell viability and blocked apoptosis in ISO exposed H9c2 cells. Moreover, Nos. 1 and 5 extracts blocked hypertrophy markers like Gαs, calcineurin, NFATc3, and BNP, thereby increasing cell proliferation markers-PI3K and AKT in a dose dependent manner. In contrast, apoptotic proteins, such as caspase-3 and cytochrome c were decreased in H9c2 cells treated with Nos. 1 and 5 extracts. We confirmed that the protective effect of No. 1 extract was partially mediated through the expression of ERK and p38, however, the No. 5 extract showed a protective effect via the ERK, JNK, and p38 pathways. This evidence provides new insights into the pharmacological role and therapeutic mechanism of Taiwan Mingjian Oolong Tea in heart diseases.

Original language	English
Pages (from-to)	77-86
Number of pages	10
Journal	American Journal of Chinese Medicine
Volume	44
Issue number	1
DOIs	https://doi.org/10.1142/S0192415X16500051
Publication status	Published - Feb 1 2016

Keywords

Cardiac Apoptosis
Cardiac Hypertrophy
Isoproterenol
Oolong Tea

ASJC Scopus subject areas

Complementary and alternative medicine

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10.1142/S0192415X16500051

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title = "Mechanism of Taiwan Mingjian Oolong Tea to Inhibit Isoproterenol-Induced Hypertrophy and Apoptosis in Cardiomyoblasts",

abstract = "This study investigates the cardio-protective effect of Nos. 1 and 5 extracts from Taiwan Mingjian Oolong Tea on H9c2 cardiomyoblast cells treated with isoproterenol (ISO). Treatment with Nos. 1 and 5 extracts increased cell viability and blocked apoptosis in ISO exposed H9c2 cells. Moreover, Nos. 1 and 5 extracts blocked hypertrophy markers like Gαs, calcineurin, NFATc3, and BNP, thereby increasing cell proliferation markers-PI3K and AKT in a dose dependent manner. In contrast, apoptotic proteins, such as caspase-3 and cytochrome c were decreased in H9c2 cells treated with Nos. 1 and 5 extracts. We confirmed that the protective effect of No. 1 extract was partially mediated through the expression of ERK and p38, however, the No. 5 extract showed a protective effect via the ERK, JNK, and p38 pathways. This evidence provides new insights into the pharmacological role and therapeutic mechanism of Taiwan Mingjian Oolong Tea in heart diseases.",

keywords = "Cardiac Apoptosis, Cardiac Hypertrophy, Isoproterenol, Oolong Tea",

author = "Yeh, {Yu Lan} and Tsai, {Hsiang I.} and Cheng, {Shiu Min} and Peiying Pai and Ho, {Tsung Jung} and Chen, {Ray Jade} and Lai, {Chao Hung} and Huang, {Pei Jane} and Padma, {V. Vijaya} and Huang, {Chih Yang}",

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doi = "10.1142/S0192415X16500051",

language = "English",

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AU - Yeh, Yu Lan

AU - Tsai, Hsiang I.

AU - Cheng, Shiu Min

AU - Pai, Peiying

AU - Ho, Tsung Jung

AU - Chen, Ray Jade

AU - Lai, Chao Hung

AU - Huang, Pei Jane

AU - Padma, V. Vijaya

AU - Huang, Chih Yang

PY - 2016/2/1

Y1 - 2016/2/1

N2 - This study investigates the cardio-protective effect of Nos. 1 and 5 extracts from Taiwan Mingjian Oolong Tea on H9c2 cardiomyoblast cells treated with isoproterenol (ISO). Treatment with Nos. 1 and 5 extracts increased cell viability and blocked apoptosis in ISO exposed H9c2 cells. Moreover, Nos. 1 and 5 extracts blocked hypertrophy markers like Gαs, calcineurin, NFATc3, and BNP, thereby increasing cell proliferation markers-PI3K and AKT in a dose dependent manner. In contrast, apoptotic proteins, such as caspase-3 and cytochrome c were decreased in H9c2 cells treated with Nos. 1 and 5 extracts. We confirmed that the protective effect of No. 1 extract was partially mediated through the expression of ERK and p38, however, the No. 5 extract showed a protective effect via the ERK, JNK, and p38 pathways. This evidence provides new insights into the pharmacological role and therapeutic mechanism of Taiwan Mingjian Oolong Tea in heart diseases.

AB - This study investigates the cardio-protective effect of Nos. 1 and 5 extracts from Taiwan Mingjian Oolong Tea on H9c2 cardiomyoblast cells treated with isoproterenol (ISO). Treatment with Nos. 1 and 5 extracts increased cell viability and blocked apoptosis in ISO exposed H9c2 cells. Moreover, Nos. 1 and 5 extracts blocked hypertrophy markers like Gαs, calcineurin, NFATc3, and BNP, thereby increasing cell proliferation markers-PI3K and AKT in a dose dependent manner. In contrast, apoptotic proteins, such as caspase-3 and cytochrome c were decreased in H9c2 cells treated with Nos. 1 and 5 extracts. We confirmed that the protective effect of No. 1 extract was partially mediated through the expression of ERK and p38, however, the No. 5 extract showed a protective effect via the ERK, JNK, and p38 pathways. This evidence provides new insights into the pharmacological role and therapeutic mechanism of Taiwan Mingjian Oolong Tea in heart diseases.

KW - Cardiac Apoptosis

KW - Cardiac Hypertrophy

KW - Isoproterenol

KW - Oolong Tea

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Mechanism of Taiwan Mingjian Oolong Tea to Inhibit Isoproterenol-Induced Hypertrophy and Apoptosis in Cardiomyoblasts

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Keywords

ASJC Scopus subject areas

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