Measurement of testosterone in seminal plasma, saliva and serum by solid-phase enzymeimmunoassay.

C. C. Shieh; S. C. Chang; C. R. Tzeng; J. J. Huang; W. J. Nir; C. Y. Hong

Measurement of testosterone in seminal plasma, saliva and serum by solid-phase enzymeimmunoassay.

C. C. Shieh, S. C. Chang, C. R. Tzeng, J. J. Huang, W. J. Nir, C. Y. Hong

Research output: Contribution to journal › Article › peer-review

Abstract

When testosterone concentrations in serum, saliva and seminal plasma were measured with a solid-phase enzymeimmunoassay in which antitestosterone antibody was bound to CNBr-Sepharose-4B, we found the ratios of testosterone in saliva and seminal plasma to that in serum were 2.5 +/- 0.2% and 5.19 +/- 0.42%. Testosterone level in saliva could be an index of free serum testosterone. In addition, we found a correlation between sperm motility and testosterone level in seminal plasma when sperm motility was measured with a transmembrane migration method. This enzymeimmunoassay could be applied to clinical studies of testosterone.

Original language	English
Pages (from-to)	614-619
Number of pages	6
Journal	Andrologia
Volume	19
Issue number	6
Publication status	Published - Nov 1987
Externally published	Yes

ASJC Scopus subject areas

Endocrinology
Nephrology

Cite this

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title = "Measurement of testosterone in seminal plasma, saliva and serum by solid-phase enzymeimmunoassay.",

abstract = "When testosterone concentrations in serum, saliva and seminal plasma were measured with a solid-phase enzymeimmunoassay in which antitestosterone antibody was bound to CNBr-Sepharose-4B, we found the ratios of testosterone in saliva and seminal plasma to that in serum were 2.5 +/- 0.2% and 5.19 +/- 0.42%. Testosterone level in saliva could be an index of free serum testosterone. In addition, we found a correlation between sperm motility and testosterone level in seminal plasma when sperm motility was measured with a transmembrane migration method. This enzymeimmunoassay could be applied to clinical studies of testosterone.",

author = "Shieh, {C. C.} and Chang, {S. C.} and Tzeng, {C. R.} and Huang, {J. J.} and Nir, {W. J.} and Hong, {C. Y.}",

year = "1987",

month = nov,

language = "English",

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AU - Shieh, C. C.

AU - Chang, S. C.

AU - Tzeng, C. R.

AU - Huang, J. J.

AU - Nir, W. J.

AU - Hong, C. Y.

PY - 1987/11

Y1 - 1987/11

N2 - When testosterone concentrations in serum, saliva and seminal plasma were measured with a solid-phase enzymeimmunoassay in which antitestosterone antibody was bound to CNBr-Sepharose-4B, we found the ratios of testosterone in saliva and seminal plasma to that in serum were 2.5 +/- 0.2% and 5.19 +/- 0.42%. Testosterone level in saliva could be an index of free serum testosterone. In addition, we found a correlation between sperm motility and testosterone level in seminal plasma when sperm motility was measured with a transmembrane migration method. This enzymeimmunoassay could be applied to clinical studies of testosterone.

AB - When testosterone concentrations in serum, saliva and seminal plasma were measured with a solid-phase enzymeimmunoassay in which antitestosterone antibody was bound to CNBr-Sepharose-4B, we found the ratios of testosterone in saliva and seminal plasma to that in serum were 2.5 +/- 0.2% and 5.19 +/- 0.42%. Testosterone level in saliva could be an index of free serum testosterone. In addition, we found a correlation between sperm motility and testosterone level in seminal plasma when sperm motility was measured with a transmembrane migration method. This enzymeimmunoassay could be applied to clinical studies of testosterone.

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VL - 19

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JO - Andrologia

JF - Andrologia

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ER -

Measurement of testosterone in seminal plasma, saliva and serum by solid-phase enzymeimmunoassay.

Abstract

ASJC Scopus subject areas

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