TY - JOUR
T1 - Measurement of lipid droplets in peripheral immune cells shows an immunomodulatory effect on monocyte polarization in experimental dyslipidaemia
AU - Hung, Yu Ping
AU - Chen, Chia Ling
AU - Tseng, Po Chun
AU - Satria, Rahmat Dani
AU - Chen, Mei Chieh
AU - Lin, Chiou Feng
N1 - Funding Information:
This work was supported by the National Science and Technology Council ( NSTC109-2327-B-006-010 , 110-2327-B-006-005 , and 110-2320-B-038-064-MY3 ), Taiwan and the Industry-University cooperation program A-111-101 .
Publisher Copyright:
© 2023 Elsevier Inc.
PY - 2023/4/2
Y1 - 2023/4/2
N2 - Intracellular lipid droplet (LD) generation is the primary site of energy storage, which is necessary for physiological homeostasis but is related to pathological metabolic disorders. Lipid metabolism is critical for maintaining innate and adaptive immunity; however, it is mainly undefined in peripheral immune cells. Flow cytometry-based immune profiling in healthy peripheral blood cells showed significant original generation of LDs in dendritic cells (DCs, CD3−CD19−CD56−CD11+), monocytes (CD3−CD19−CD56−CD14+), natural killer cells (NK, CD3−CD19−CD56+), and B cells (CD3−CD19+). CD36, a common scavenger receptor of lipids, was also highly expressed in LD-accumulated DCs and monocytes. Following short-term treatment with oxidized LDL (oxLDL) in an experimental ex vivo model, CD14+ monocytes showed an effective increase in LD generation, but there were no alterations in the immune cell populations. Furthermore, oxLDL-treated CD14+ monocytes displayed CD36 expression. However, oxLDL-primed CD14+ monocytes showed a blockade in the uptake of extra oxLDL, even while expressing increased CD36, indicating a defect in lipid clearance. Exogenous treatment with oxLDL caused monocyte type 1 polarization accompanied by increased LD accumulation and CD36 expression. This study describes a method to monitor LD generation and CD36 expression in peripheral immune cells and identified an immunomodulatory effect of oxLDL on monocytes by tilting them towards type 1 polarization.
AB - Intracellular lipid droplet (LD) generation is the primary site of energy storage, which is necessary for physiological homeostasis but is related to pathological metabolic disorders. Lipid metabolism is critical for maintaining innate and adaptive immunity; however, it is mainly undefined in peripheral immune cells. Flow cytometry-based immune profiling in healthy peripheral blood cells showed significant original generation of LDs in dendritic cells (DCs, CD3−CD19−CD56−CD11+), monocytes (CD3−CD19−CD56−CD14+), natural killer cells (NK, CD3−CD19−CD56+), and B cells (CD3−CD19+). CD36, a common scavenger receptor of lipids, was also highly expressed in LD-accumulated DCs and monocytes. Following short-term treatment with oxidized LDL (oxLDL) in an experimental ex vivo model, CD14+ monocytes showed an effective increase in LD generation, but there were no alterations in the immune cell populations. Furthermore, oxLDL-treated CD14+ monocytes displayed CD36 expression. However, oxLDL-primed CD14+ monocytes showed a blockade in the uptake of extra oxLDL, even while expressing increased CD36, indicating a defect in lipid clearance. Exogenous treatment with oxLDL caused monocyte type 1 polarization accompanied by increased LD accumulation and CD36 expression. This study describes a method to monitor LD generation and CD36 expression in peripheral immune cells and identified an immunomodulatory effect of oxLDL on monocytes by tilting them towards type 1 polarization.
KW - CD36
KW - Immune cells
KW - Lipid droplets
KW - Monocyte polarization
KW - OxLDL
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U2 - 10.1016/j.bbrc.2023.02.006
DO - 10.1016/j.bbrc.2023.02.006
M3 - Article
AN - SCOPUS:85147812789
SN - 0006-291X
VL - 650
SP - 73
EP - 80
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
ER -