Mandibular alveolar bony defect repair using bone morphogenetic protein 2-expressing autologous mesenchymal stem cells

Vanessa Hui Ying Chung, Amy Yi Ling Chen, Chang Chin Kwan, Philip Kao Ting Chen, Sophia Chia Ning Chang

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)


BACKGROUND:: Mandibular bone regeneration is stepped up by human recombinant bone morphogenetic protein 2 (BMP-2) whose application is also related to limited cementum and periodontal ligament regeneration, local root resorption, and ankylosis. The alveolar bone grafting without traditional autologous bone grafts remains a challenge for plastic surgeons. METHODS:: Bilateral mandibular alveolar and periodontal defects were created over the premolar areas in 9 mature male beagles. The defects were randomly assigned for either the adenovirus BMP-2 (advBMP-2) group with BMP-2-expressing mesenchymal stem cells (MSCs) or the control with MSCs alone. The regenerated periodontal attachment apparatus was evaluated histologically, and the whole regenerated bone volume was scrutinized from three-dimensional computed tomography analysis. RESULTS:: Periodontal apparatus regeneration was significantly better in the advBMP-2 group. New cementum and Sharpey fibers were observed on the denuded root surfaces in the advBMP-2 group, whereas incomplete healing with localized root surface resorption was noted in the control group. Eight weeks after implantation, the advBMP-2 group showed significant increase in bone regeneration than the control one. CONCLUSIONS:: Thus, the use of ex vivo BMP-2-engineered autologous MSCs boosted bone and periodontal apparatus regeneration in mandibular periodontal defects. This de novo approach might be suitable for clinical mandibular bone repair and periodontal apparatus repair.

Original languageEnglish
Pages (from-to)450-454
Number of pages5
JournalJournal of Craniofacial Surgery
Issue number2
Publication statusPublished - Mar 2011
Externally publishedYes


  • bone marrow stem cells
  • bone morphogenetic protein
  • ex vivo gene therapy
  • mesenchymal stem cells
  • Periodontal regeneration
  • regional cell therapy
  • root ankylosis
  • root resorption
  • Sharpey fiber
  • tissue engineering

ASJC Scopus subject areas

  • Surgery
  • Otorhinolaryngology


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