Magnetic-Composite-Modified Polycrystalline Silicon Nanowire Field-Effect Transistor for Vascular Endothelial Growth Factor Detection and Cancer Diagnosis

Hsiao Chien Chen; Jian Tai Qiu; Fu Liang Yang; Yin Chih Liu; Min Cheng Chen; Rung Ywan Tsai; Hung Wei Yang; Chia Yi Lin; Chu Chi Lin; Tzong Shoon Wu; Yi Ming Tu; Min Cong Xiao; Chia Hua Ho; Chien Chao Huang; Chao Sung Lai; Mu Yi Hua

doi:10.1021/ac5001898

Magnetic-Composite-Modified Polycrystalline Silicon Nanowire Field-Effect Transistor for Vascular Endothelial Growth Factor Detection and Cancer Diagnosis

Hsiao Chien Chen, Jian Tai Qiu, Fu Liang Yang, Yin Chih Liu, Min Cheng Chen, Rung Ywan Tsai, Hung Wei Yang, Chia Yi Lin, Chu Chi Lin, Tzong Shoon Wu, Yi Ming Tu, Min Cong Xiao, Chia Hua Ho, Chien Chao Huang, Chao Sung Lai, Mu Yi Hua

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

This study proposes a vascular endothelial growth factor (VEGF) biosensor for diagnosing various stages of cervical carcinoma. In addition, VEGF concentrations at various stages of cancer therapy are determined and compared to data obtained by computed tomography (CT) and cancer antigen 125 (CA-125). The increase in VEGF concentrations during operations offers useful insight into dosage timing during cancer therapy. This biosensor uses Avastin as the biorecognition element for the potential cancer biomarker VEGF and is based on a n-type polycrystalline silicon nanowire field-effect transistor (poly-SiNW-FET). Magnetic nanoparticles with poly[aniline-co-N-(1-one-butyric acid) aniline]-Fe₃O₄ (SPAnH-Fe₃O₄) shell-core structures are used as carriers for Avastin loading and provide rapid purification due to their magnetic properties, which prevent the loss of bioactivity; furthermore, the high surface area of these structures increases the quantity of Avastin immobilized. Average concentrations in human blood for species that interfere with detection specificity are also evaluated. The detection range of the biosensor for serum samples covers the results expected from both healthy individuals and cancer patients.

Original language	English
Pages (from-to)	9443-9450
Number of pages	8
Journal	Analytical Chemistry
Volume	86
Issue number	19
DOIs	https://doi.org/10.1021/ac5001898
Publication status	Published - Oct 7 2014
Externally published	Yes

ASJC Scopus subject areas

Analytical Chemistry

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1021/ac5001898

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Chen, H. C., Qiu, J. T., Yang, F. L., Liu, Y. C., Chen, M. C., Tsai, R. Y., Yang, H. W., Lin, C. Y., Lin, C. C., Wu, T. S., Tu, Y. M., Xiao, M. C., Ho, C. H., Huang, C. C., Lai, C. S., & Hua, M. Y. (2014). Magnetic-Composite-Modified Polycrystalline Silicon Nanowire Field-Effect Transistor for Vascular Endothelial Growth Factor Detection and Cancer Diagnosis. Analytical Chemistry, 86(19), 9443-9450. https://doi.org/10.1021/ac5001898

Chen, HC, Qiu, JT, Yang, FL, Liu, YC, Chen, MC, Tsai, RY, Yang, HW, Lin, CY, Lin, CC, Wu, TS, Tu, YM, Xiao, MC, Ho, CH, Huang, CC, Lai, CS & Hua, MY 2014, 'Magnetic-Composite-Modified Polycrystalline Silicon Nanowire Field-Effect Transistor for Vascular Endothelial Growth Factor Detection and Cancer Diagnosis', Analytical Chemistry, vol. 86, no. 19, pp. 9443-9450. https://doi.org/10.1021/ac5001898

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title = "Magnetic-Composite-Modified Polycrystalline Silicon Nanowire Field-Effect Transistor for Vascular Endothelial Growth Factor Detection and Cancer Diagnosis",

abstract = "This study proposes a vascular endothelial growth factor (VEGF) biosensor for diagnosing various stages of cervical carcinoma. In addition, VEGF concentrations at various stages of cancer therapy are determined and compared to data obtained by computed tomography (CT) and cancer antigen 125 (CA-125). The increase in VEGF concentrations during operations offers useful insight into dosage timing during cancer therapy. This biosensor uses Avastin as the biorecognition element for the potential cancer biomarker VEGF and is based on a n-type polycrystalline silicon nanowire field-effect transistor (poly-SiNW-FET). Magnetic nanoparticles with poly[aniline-co-N-(1-one-butyric acid) aniline]-Fe3O4 (SPAnH-Fe3O4) shell-core structures are used as carriers for Avastin loading and provide rapid purification due to their magnetic properties, which prevent the loss of bioactivity; furthermore, the high surface area of these structures increases the quantity of Avastin immobilized. Average concentrations in human blood for species that interfere with detection specificity are also evaluated. The detection range of the biosensor for serum samples covers the results expected from both healthy individuals and cancer patients.",

author = "Chen, {Hsiao Chien} and Qiu, {Jian Tai} and Yang, {Fu Liang} and Liu, {Yin Chih} and Chen, {Min Cheng} and Tsai, {Rung Ywan} and Yang, {Hung Wei} and Lin, {Chia Yi} and Lin, {Chu Chi} and Wu, {Tzong Shoon} and Tu, {Yi Ming} and Xiao, {Min Cong} and Ho, {Chia Hua} and Huang, {Chien Chao} and Lai, {Chao Sung} and Hua, {Mu Yi}",

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year = "2014",

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doi = "10.1021/ac5001898",

language = "English",

volume = "86",

pages = "9443--9450",

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AU - Chen, Hsiao Chien

AU - Qiu, Jian Tai

AU - Yang, Fu Liang

AU - Liu, Yin Chih

AU - Chen, Min Cheng

AU - Tsai, Rung Ywan

AU - Yang, Hung Wei

AU - Lin, Chia Yi

AU - Lin, Chu Chi

AU - Wu, Tzong Shoon

AU - Tu, Yi Ming

AU - Xiao, Min Cong

AU - Ho, Chia Hua

AU - Huang, Chien Chao

AU - Lai, Chao Sung

AU - Hua, Mu Yi

PY - 2014/10/7

Y1 - 2014/10/7

N2 - This study proposes a vascular endothelial growth factor (VEGF) biosensor for diagnosing various stages of cervical carcinoma. In addition, VEGF concentrations at various stages of cancer therapy are determined and compared to data obtained by computed tomography (CT) and cancer antigen 125 (CA-125). The increase in VEGF concentrations during operations offers useful insight into dosage timing during cancer therapy. This biosensor uses Avastin as the biorecognition element for the potential cancer biomarker VEGF and is based on a n-type polycrystalline silicon nanowire field-effect transistor (poly-SiNW-FET). Magnetic nanoparticles with poly[aniline-co-N-(1-one-butyric acid) aniline]-Fe3O4 (SPAnH-Fe3O4) shell-core structures are used as carriers for Avastin loading and provide rapid purification due to their magnetic properties, which prevent the loss of bioactivity; furthermore, the high surface area of these structures increases the quantity of Avastin immobilized. Average concentrations in human blood for species that interfere with detection specificity are also evaluated. The detection range of the biosensor for serum samples covers the results expected from both healthy individuals and cancer patients.

AB - This study proposes a vascular endothelial growth factor (VEGF) biosensor for diagnosing various stages of cervical carcinoma. In addition, VEGF concentrations at various stages of cancer therapy are determined and compared to data obtained by computed tomography (CT) and cancer antigen 125 (CA-125). The increase in VEGF concentrations during operations offers useful insight into dosage timing during cancer therapy. This biosensor uses Avastin as the biorecognition element for the potential cancer biomarker VEGF and is based on a n-type polycrystalline silicon nanowire field-effect transistor (poly-SiNW-FET). Magnetic nanoparticles with poly[aniline-co-N-(1-one-butyric acid) aniline]-Fe3O4 (SPAnH-Fe3O4) shell-core structures are used as carriers for Avastin loading and provide rapid purification due to their magnetic properties, which prevent the loss of bioactivity; furthermore, the high surface area of these structures increases the quantity of Avastin immobilized. Average concentrations in human blood for species that interfere with detection specificity are also evaluated. The detection range of the biosensor for serum samples covers the results expected from both healthy individuals and cancer patients.

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Magnetic-Composite-Modified Polycrystalline Silicon Nanowire Field-Effect Transistor for Vascular Endothelial Growth Factor Detection and Cancer Diagnosis

Abstract

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UN SDGs

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