Low incidence of BCL-6 gene alterations for diffuse large B-cell lymphomas in Taiwan Chinese

Po Min Chen, Muh Hwa Yang, I. Ting Yu, Jen Tsun Lin, Yu Chen Lin, Frank S. Fan, Wei Shu Wang, Chueh Chuan Yen, Tzeon Jye Chiou, Jin Hwang Liu

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)

Abstract

BACKGROUND. In Western populations, rearrangement of the BCL-6 gene can be identified in 20-40% of patients with diffuse large B-cell lymphoma (DLBCL). Analysis of the BCL-6 gene has revealed the presence of point mutations or small deletions in 70% of DLBCL tumors; however, few studies have investigated BCL-6 gene alteration in patients with non-Hodgkins lymphoma (NHL) of Chinese descent. METHODS. Samples from 135 Taiwanese patients with NHL were examined (28 samples of T-cell NHL and 107 samples of B-cell NHL; 59 samples from patients with DLBCL) for gene rearrangement and mutation of the BCL-6 proto-oncogene using Southern blot analysis and single-strand conformation polymorphism (SSCP) followed by sequence analysis. RESULTS. BCL-6 rearrangement and point mutations were found in 14.8% of patients (n = 20) and in 7.4% of patients (n = 10), respectively. All BCL-6 gene alterations occurred in patients with B-cell NHL, and none occurred in patients with T-cell NHL. Among the 59 patients with DLBCL, BCL-6 gene rearrangements were identified in 10 patients (16.9%), and mutations were identified in 8 patients (13.6%), with the BCL-6 mutation occurring independent of the BCL-6 rearrangement. The incidence of BCL-6 gene rearrangement and mutations in patients with extranodal DLBCL was 9.5% (2 of 21 patients) and 23.8% (5 of 21 patients), respectively. Univariate analysis and multivariate logistic regression found no association between BCL-6 gene alternations and clinical characteristics, including extranodal tumors in patients with DLBCL, and no association between the BCL-6 alterations and prognosis was found. CONCLUSIONS. The incidence of BCL-6 alterations was lower in Taiwanese patients with DLBCL compared with Western populations, and BCL-6 gene alterations showed no prognostic significance in patients with DLBCL.

Original languageEnglish
Pages (from-to)2635-2644
Number of pages10
JournalCancer
Volume94
Issue number10
DOIs
Publication statusPublished - May 2002
Externally publishedYes

Keywords

  • BCL-6
  • Diffuse large B-cell lymphoma
  • Gene rearrangement
  • Mutation

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Fingerprint

Dive into the research topics of 'Low incidence of BCL-6 gene alterations for diffuse large B-cell lymphomas in Taiwan Chinese'. Together they form a unique fingerprint.

Cite this