Localization and characterization of an orphan receptor, guanylyl cyclase-G, in mouse testis and sperm

Yen Hua Huang, Chih Chun Wei, Yueh Hsing Su, Bo Tsung Wu, Yi Yun Ciou, Cheng Fen Tu, Trevor G. Cooper, Ching Hei Yeung, Sin Tak Chu, Ming Tzu Tsai, Ruey Bing Yang

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)


We recently identified a novel testis-enriched receptor guanylyl cyclase (GC) in the mouse, designated mGC-G. To further investigate its protein expression and function, we generated a neutralizing antibody specifically against the extracellular domain of this receptor. RT-PCR and immunohistochemical analyses show that mGC-G is predominantly expressed from round spermatids to spermatozoa in mouse testis at both the mRNA and protein levels. Flow cytometry and confocal immunofluorescence reveal that mGC-G is a cell surface protein restricted to the plasma membrane overlying the acrosome and midpiece of the flagellum in mature sperm. Interestingly, Western blot analysis demonstrates that testicular mGC-G is approximately 180 kDa but is subject to limited proteolysis during epididymal sperm transport, resulting in a smaller fragment tethered on the mature sperm surface. On Fluo-3 cytometrical analysis and computer-assisted sperm assay, we found that serum albumin-induced elevation of sperm intracellular Ca2+ concentration, protein tyrosine phosphorylation, and progressive motility associated with capacitation are markedly reduced by preincubation of the anti-mGC-G neutralizing antibody. Together, these results indicate that mGC-G is proteolytically modified in mature sperm membrane and suggest that mGC-G-mediated signaling may play a critical role in gamete/reproductive biology.

Original languageEnglish
Pages (from-to)4792-4800
Number of pages9
Issue number10
Publication statusPublished - 2006

ASJC Scopus subject areas

  • Endocrinology


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