LMP1 gene detection through polymerase chain reaction in microfluidic chip combined with nanoslit surface plasmon resonance sensor

Han Yun Hsieh; Ray Chang; Yung Yu Huang; Pei Kuen Wei; Yu Jui Fan; Horn Jiunn Sheen

LMP1 gene detection through polymerase chain reaction in microfluidic chip combined with nanoslit surface plasmon resonance sensor

Han Yun Hsieh
, Ray Chang
, Yung Yu Huang
, Pei Kuen Wei
, Yu Jui Fan
, Horn Jiunn Sheen

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Latent membrane protein 1 (LMP1), one of the main performance proteins of the Epstein-Barr virus (EBV), also highly associated with Nasopharyngeal carcinoma (NPC). Herein, a combined microfluidic polymerase chain reaction (PCR) device with nanoslit surface plasmon resonance (SPR) sensor for LMP1 DNA detection has developed, enabling the amplification and detection of the LMP1 gene on a combined platform. With 30 cycle PCR amplification, the detection limit of 10 pg/mL can be found. Moreover, extracted DNA from EBV-positive cells could be detected through the device, showing the potential of clinical diagnosis. This integrated device performed a label-free, real-time, highly sensitive, low cost, and rapid detection.

Original language	English
Title of host publication	MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Publisher	Chemical and Biological Microsystems Society
Pages	725-726
Number of pages	2
ISBN (Electronic)	9781733419031
Publication status	Published - 2021
Event	25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021 - Palm Springs, Virtual, United States Duration: Oct 10 2021 → Oct 14 2021

Publication series

Name	MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Conference

Conference	25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021
Country/Territory	United States
City	Palm Springs, Virtual
Period	10/10/21 → 10/14/21

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Epstein-Barr virus (EBV)
microfluidics
polymerase chain reaction
surface plasmon resonance

ASJC Scopus subject areas

Bioengineering
Chemical Engineering (miscellaneous)

Cite this

Hsieh, H. Y., Chang, R., Huang, Y. Y., Wei, P. K., Fan, Y. J., & Sheen, H. J. (2021). LMP1 gene detection through polymerase chain reaction in microfluidic chip combined with nanoslit surface plasmon resonance sensor. In MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 725-726). (MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences). Chemical and Biological Microsystems Society.

LMP1 gene detection through polymerase chain reaction in microfluidic chip combined with nanoslit surface plasmon resonance sensor. / Hsieh, Han Yun; Chang, Ray; Huang, Yung Yu et al.
MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2021. p. 725-726 (MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Hsieh, HY, Chang, R, Huang, YY, Wei, PK, Fan, YJ & Sheen, HJ 2021, LMP1 gene detection through polymerase chain reaction in microfluidic chip combined with nanoslit surface plasmon resonance sensor. in MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, Chemical and Biological Microsystems Society, pp. 725-726, 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021, Palm Springs, Virtual, United States, 10/10/21.

Hsieh HY, Chang R, Huang YY, Wei PK, Fan YJ, Sheen HJ. LMP1 gene detection through polymerase chain reaction in microfluidic chip combined with nanoslit surface plasmon resonance sensor. In MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society. 2021. p. 725-726. (MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences).

Hsieh, Han Yun ; Chang, Ray ; Huang, Yung Yu et al. / LMP1 gene detection through polymerase chain reaction in microfluidic chip combined with nanoslit surface plasmon resonance sensor. MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2021. pp. 725-726 (MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences).

@inproceedings{3c7c6e9982ba45a28ec93bfdc02b3ffa,

title = "LMP1 gene detection through polymerase chain reaction in microfluidic chip combined with nanoslit surface plasmon resonance sensor",

abstract = "Latent membrane protein 1 (LMP1), one of the main performance proteins of the Epstein-Barr virus (EBV), also highly associated with Nasopharyngeal carcinoma (NPC). Herein, a combined microfluidic polymerase chain reaction (PCR) device with nanoslit surface plasmon resonance (SPR) sensor for LMP1 DNA detection has developed, enabling the amplification and detection of the LMP1 gene on a combined platform. With 30 cycle PCR amplification, the detection limit of 10 pg/mL can be found. Moreover, extracted DNA from EBV-positive cells could be detected through the device, showing the potential of clinical diagnosis. This integrated device performed a label-free, real-time, highly sensitive, low cost, and rapid detection.",

keywords = "Epstein-Barr virus (EBV), microfluidics, polymerase chain reaction, surface plasmon resonance",

author = "Hsieh, \{Han Yun\} and Ray Chang and Huang, \{Yung Yu\} and Wei, \{Pei Kuen\} and Fan, \{Yu Jui\} and Sheen, \{Horn Jiunn\}",

note = "Funding Information: This paper is supported by the Ministry of Science and Technology in Taiwan, MOST108-2221-E-002-052. REFERENCES [1] M. A. Epstein, B. G. Achong and Y. M. Barr: Virus particles in cultured lymphoblasts from burkitt's lymphoma. Lancet, 1(7335), 702-3 (1964) [2] Y. P. Chen, W. N. Zhang, L. Chen, L. L. Tang, Y. P. Mao, W. F. Li, X. Liu, G. Q. Zhou, Y. Sun, T. B. Kang, M. S. Zeng, N. Liu and J. Ma: Effect of latent membrane protein 1 expression on overall survival in Epstein-Barr virus-associated cancers: a literature-based meta-analysis. Oncotarget, 6(30), 29311-29323 (2015) [3] J. L. Ryan, H. Fan, S. L. Glaser, S. A. Schichman, N. Raab-Traub and M. L. Gulley: Epstein-Barr Virus Quantitation by Real-Time PCR Targeting Multiple Gene Segments: A Novel Approach to Screen for the Virus in Paraffin-Embedded Tissue and Plasma. The Journal of Molecular Diagnostics, 6(4), 378-385 (2004) [4] X.-M. Li, W.-H. Xiao and H.-X. Zhao: Prognostic significance of latent membrane protein 1 expression in non-Hodgkin lymphoma: A meta-analysis. Medicine, 96(14) (2017) CONTACT Han-Yun Hsieh: [email protected] Publisher Copyright: {\textcopyright} 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.; 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021 ; Conference date: 10-10-2021 Through 14-10-2021",

year = "2021",

language = "English",

series = "MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences",

publisher = "Chemical and Biological Microsystems Society",

pages = "725--726",

booktitle = "MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences",

}

TY - GEN

T1 - LMP1 gene detection through polymerase chain reaction in microfluidic chip combined with nanoslit surface plasmon resonance sensor

AU - Hsieh, Han Yun

AU - Chang, Ray

AU - Huang, Yung Yu

AU - Wei, Pei Kuen

AU - Fan, Yu Jui

AU - Sheen, Horn Jiunn

N1 - Funding Information: This paper is supported by the Ministry of Science and Technology in Taiwan, MOST108-2221-E-002-052. REFERENCES [1] M. A. Epstein, B. G. Achong and Y. M. Barr: Virus particles in cultured lymphoblasts from burkitt's lymphoma. Lancet, 1(7335), 702-3 (1964) [2] Y. P. Chen, W. N. Zhang, L. Chen, L. L. Tang, Y. P. Mao, W. F. Li, X. Liu, G. Q. Zhou, Y. Sun, T. B. Kang, M. S. Zeng, N. Liu and J. Ma: Effect of latent membrane protein 1 expression on overall survival in Epstein-Barr virus-associated cancers: a literature-based meta-analysis. Oncotarget, 6(30), 29311-29323 (2015) [3] J. L. Ryan, H. Fan, S. L. Glaser, S. A. Schichman, N. Raab-Traub and M. L. Gulley: Epstein-Barr Virus Quantitation by Real-Time PCR Targeting Multiple Gene Segments: A Novel Approach to Screen for the Virus in Paraffin-Embedded Tissue and Plasma. The Journal of Molecular Diagnostics, 6(4), 378-385 (2004) [4] X.-M. Li, W.-H. Xiao and H.-X. Zhao: Prognostic significance of latent membrane protein 1 expression in non-Hodgkin lymphoma: A meta-analysis. Medicine, 96(14) (2017) CONTACT Han-Yun Hsieh: [email protected] Publisher Copyright: © 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.

PY - 2021

Y1 - 2021

N2 - Latent membrane protein 1 (LMP1), one of the main performance proteins of the Epstein-Barr virus (EBV), also highly associated with Nasopharyngeal carcinoma (NPC). Herein, a combined microfluidic polymerase chain reaction (PCR) device with nanoslit surface plasmon resonance (SPR) sensor for LMP1 DNA detection has developed, enabling the amplification and detection of the LMP1 gene on a combined platform. With 30 cycle PCR amplification, the detection limit of 10 pg/mL can be found. Moreover, extracted DNA from EBV-positive cells could be detected through the device, showing the potential of clinical diagnosis. This integrated device performed a label-free, real-time, highly sensitive, low cost, and rapid detection.

AB - Latent membrane protein 1 (LMP1), one of the main performance proteins of the Epstein-Barr virus (EBV), also highly associated with Nasopharyngeal carcinoma (NPC). Herein, a combined microfluidic polymerase chain reaction (PCR) device with nanoslit surface plasmon resonance (SPR) sensor for LMP1 DNA detection has developed, enabling the amplification and detection of the LMP1 gene on a combined platform. With 30 cycle PCR amplification, the detection limit of 10 pg/mL can be found. Moreover, extracted DNA from EBV-positive cells could be detected through the device, showing the potential of clinical diagnosis. This integrated device performed a label-free, real-time, highly sensitive, low cost, and rapid detection.

KW - Epstein-Barr virus (EBV)

KW - microfluidics

KW - polymerase chain reaction

KW - surface plasmon resonance

UR - https://www.scopus.com/pages/publications/85136936883

UR - https://www.scopus.com/pages/publications/85136936883#tab=citedBy

M3 - Conference contribution

AN - SCOPUS:85136936883

T3 - MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences

SP - 725

EP - 726

BT - MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences

PB - Chemical and Biological Microsystems Society

T2 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021

Y2 - 10 October 2021 through 14 October 2021

ER -

LMP1 gene detection through polymerase chain reaction in microfluidic chip combined with nanoslit surface plasmon resonance sensor

Abstract

Publication series

Conference

UN SDGs

Keywords

ASJC Scopus subject areas

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Cite this