Leukocyte-endothelium interaction: Measurement by laser tweezers force spectroscopy

Shi Kang Wang; Jeng Jiann Chiu; Ming Rou Lee; Shih Chin Chou; Li Jing Chen; Ned H.C. Hwang

doi:10.1007/s10558-006-9012-6

Leukocyte-endothelium interaction: Measurement by laser tweezers force spectroscopy

Shi Kang Wang, Jeng Jiann Chiu, Ming Rou Lee, Shih Chin Chou, Li Jing Chen, Ned H.C. Hwang

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

Leukocyte adhesion to vascular endothelium is an initial step of many inflammatory diseases. Although the atomic force microscopy (AFM) measurements of leukocyte-endothelial interaction have been recently introduced. with cell adhesion force unbinding curves (CAFUC). We obtained pico-Newton force in the initial interaction between a single living THP-1 cell and HUVEC monolayer using a custom-built laser tweezers (LT) system. The measured quantities included the non-linear force-distance relationship, and the effect of yielding in cell detachment. It is possible to introduce a time scale into the LT cell-detachment experiments for further exploration and more detailed information on the viscoelastic properties of living cells.

Original language	English
Pages (from-to)	111-117
Number of pages	7
Journal	Cardiovascular Engineering
Volume	6
Issue number	3
DOIs	https://doi.org/10.1007/s10558-006-9012-6
Publication status	Published - Sept 1 2006
Externally published	Yes

Keywords

Cell adhesion
Endothelial cell
Force unbinding curve
Laser tweezers
Leukocyte
Optical trap

ASJC Scopus subject areas

Surgery
Cardiology and Cardiovascular Medicine
Transplantation

Access to Document

10.1007/s10558-006-9012-6

Cite this

@article{c65fbf8bbda34171a977cb1da4506dd5,

title = "Leukocyte-endothelium interaction: Measurement by laser tweezers force spectroscopy",

abstract = "Leukocyte adhesion to vascular endothelium is an initial step of many inflammatory diseases. Although the atomic force microscopy (AFM) measurements of leukocyte-endothelial interaction have been recently introduced. with cell adhesion force unbinding curves (CAFUC). We obtained pico-Newton force in the initial interaction between a single living THP-1 cell and HUVEC monolayer using a custom-built laser tweezers (LT) system. The measured quantities included the non-linear force-distance relationship, and the effect of yielding in cell detachment. It is possible to introduce a time scale into the LT cell-detachment experiments for further exploration and more detailed information on the viscoelastic properties of living cells.",

keywords = "Cell adhesion, Endothelial cell, Force unbinding curve, Laser tweezers, Leukocyte, Optical trap",

author = "Wang, \{Shi Kang\} and Chiu, \{Jeng Jiann\} and Lee, \{Ming Rou\} and Chou, \{Shih Chin\} and Chen, \{Li Jing\} and Hwang, \{Ned H.C.\}",

note = "Funding Information: Acknowledgements This work was supported by Grants MM-PP-09-02 which is a part of a parallel grant funded to both the Medical Engineering Division, NHRI and the Department of Bioengineering, UCSD (PI: Prof. Y. C. Fung) from the National Health Research Institutes (NHRI), Taiwan, R.O.C. Prof. SK Wang, was a visiting investigator on leave from the Tianjin University, Tianjin, P.R. China.",

year = "2006",

month = sep,

day = "1",

doi = "10.1007/s10558-006-9012-6",

language = "English",

volume = "6",

pages = "111--117",

journal = "Cardiovascular Engineering",

issn = "1567-8822",

publisher = "Springer Science + Business Media",

number = "3",

}

TY - JOUR

T1 - Leukocyte-endothelium interaction

T2 - Measurement by laser tweezers force spectroscopy

AU - Wang, Shi Kang

AU - Chiu, Jeng Jiann

AU - Lee, Ming Rou

AU - Chou, Shih Chin

AU - Chen, Li Jing

AU - Hwang, Ned H.C.

N1 - Funding Information: Acknowledgements This work was supported by Grants MM-PP-09-02 which is a part of a parallel grant funded to both the Medical Engineering Division, NHRI and the Department of Bioengineering, UCSD (PI: Prof. Y. C. Fung) from the National Health Research Institutes (NHRI), Taiwan, R.O.C. Prof. SK Wang, was a visiting investigator on leave from the Tianjin University, Tianjin, P.R. China.

PY - 2006/9/1

Y1 - 2006/9/1

N2 - Leukocyte adhesion to vascular endothelium is an initial step of many inflammatory diseases. Although the atomic force microscopy (AFM) measurements of leukocyte-endothelial interaction have been recently introduced. with cell adhesion force unbinding curves (CAFUC). We obtained pico-Newton force in the initial interaction between a single living THP-1 cell and HUVEC monolayer using a custom-built laser tweezers (LT) system. The measured quantities included the non-linear force-distance relationship, and the effect of yielding in cell detachment. It is possible to introduce a time scale into the LT cell-detachment experiments for further exploration and more detailed information on the viscoelastic properties of living cells.

AB - Leukocyte adhesion to vascular endothelium is an initial step of many inflammatory diseases. Although the atomic force microscopy (AFM) measurements of leukocyte-endothelial interaction have been recently introduced. with cell adhesion force unbinding curves (CAFUC). We obtained pico-Newton force in the initial interaction between a single living THP-1 cell and HUVEC monolayer using a custom-built laser tweezers (LT) system. The measured quantities included the non-linear force-distance relationship, and the effect of yielding in cell detachment. It is possible to introduce a time scale into the LT cell-detachment experiments for further exploration and more detailed information on the viscoelastic properties of living cells.

KW - Cell adhesion

KW - Endothelial cell

KW - Force unbinding curve

KW - Laser tweezers

KW - Leukocyte

KW - Optical trap

UR - https://www.scopus.com/pages/publications/33751027762

UR - https://www.scopus.com/inward/citedby.url?scp=33751027762&partnerID=8YFLogxK

U2 - 10.1007/s10558-006-9012-6

DO - 10.1007/s10558-006-9012-6

M3 - Article

C2 - 16960761

AN - SCOPUS:33751027762

SN - 1567-8822

VL - 6

SP - 111

EP - 117

JO - Cardiovascular Engineering

JF - Cardiovascular Engineering

IS - 3

ER -

Leukocyte-endothelium interaction: Measurement by laser tweezers force spectroscopy

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this