Leukocyte-endothelium interaction: Measurement by laser tweezers force spectroscopy

Shi Kang Wang, Jeng Jiann Chiu, Ming Rou Lee, Shih Chin Chou, Li Jing Chen, Ned H.C. Hwang

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)


Leukocyte adhesion to vascular endothelium is an initial step of many inflammatory diseases. Although the atomic force microscopy (AFM) measurements of leukocyte-endothelial interaction have been recently introduced. with cell adhesion force unbinding curves (CAFUC). We obtained pico-Newton force in the initial interaction between a single living THP-1 cell and HUVEC monolayer using a custom-built laser tweezers (LT) system. The measured quantities included the non-linear force-distance relationship, and the effect of yielding in cell detachment. It is possible to introduce a time scale into the LT cell-detachment experiments for further exploration and more detailed information on the viscoelastic properties of living cells.

Original languageEnglish
Pages (from-to)111-117
Number of pages7
JournalCardiovascular Engineering
Issue number3
Publication statusPublished - Sept 1 2006
Externally publishedYes


  • Cell adhesion
  • Endothelial cell
  • Force unbinding curve
  • Laser tweezers
  • Leukocyte
  • Optical trap

ASJC Scopus subject areas

  • Surgery
  • Cardiology and Cardiovascular Medicine
  • Transplantation


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