Leukocyte-endothelium interaction: Measurement by laser tweezers force spectroscopy

Shi Kang Wang; Jeng Jiann Chiu; Ming Rou Lee; Shih Chin Chou; Li Jing Chen; Ned H.C. Hwang

doi:10.1007/s10558-006-9012-6

Leukocyte-endothelium interaction: Measurement by laser tweezers force spectroscopy

Shi Kang Wang, Jeng Jiann Chiu, Ming Rou Lee, Shih Chin Chou, Li Jing Chen, Ned H.C. Hwang

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

Leukocyte adhesion to vascular endothelium is an initial step of many inflammatory diseases. Although the atomic force microscopy (AFM) measurements of leukocyte-endothelial interaction have been recently introduced. with cell adhesion force unbinding curves (CAFUC). We obtained pico-Newton force in the initial interaction between a single living THP-1 cell and HUVEC monolayer using a custom-built laser tweezers (LT) system. The measured quantities included the non-linear force-distance relationship, and the effect of yielding in cell detachment. It is possible to introduce a time scale into the LT cell-detachment experiments for further exploration and more detailed information on the viscoelastic properties of living cells.

Original language	English
Pages (from-to)	111-117
Number of pages	7
Journal	Cardiovascular Engineering
Volume	6
Issue number	3
DOIs	https://doi.org/10.1007/s10558-006-9012-6
Publication status	Published - Sept 1 2006
Externally published	Yes

Keywords

Cell adhesion
Endothelial cell
Force unbinding curve
Laser tweezers
Leukocyte
Optical trap

ASJC Scopus subject areas

Surgery
Cardiology and Cardiovascular Medicine
Transplantation

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10.1007/s10558-006-9012-6

Cite this

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title = "Leukocyte-endothelium interaction: Measurement by laser tweezers force spectroscopy",

abstract = "Leukocyte adhesion to vascular endothelium is an initial step of many inflammatory diseases. Although the atomic force microscopy (AFM) measurements of leukocyte-endothelial interaction have been recently introduced. with cell adhesion force unbinding curves (CAFUC). We obtained pico-Newton force in the initial interaction between a single living THP-1 cell and HUVEC monolayer using a custom-built laser tweezers (LT) system. The measured quantities included the non-linear force-distance relationship, and the effect of yielding in cell detachment. It is possible to introduce a time scale into the LT cell-detachment experiments for further exploration and more detailed information on the viscoelastic properties of living cells.",

keywords = "Cell adhesion, Endothelial cell, Force unbinding curve, Laser tweezers, Leukocyte, Optical trap",

author = "Wang, {Shi Kang} and Chiu, {Jeng Jiann} and Lee, {Ming Rou} and Chou, {Shih Chin} and Chen, {Li Jing} and Hwang, {Ned H.C.}",

note = "Funding Information: Acknowledgements This work was supported by Grants MM-PP-09-02 which is a part of a parallel grant funded to both the Medical Engineering Division, NHRI and the Department of Bioengineering, UCSD (PI: Prof. Y. C. Fung) from the National Health Research Institutes (NHRI), Taiwan, R.O.C. Prof. SK Wang, was a visiting investigator on leave from the Tianjin University, Tianjin, P.R. China.",

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doi = "10.1007/s10558-006-9012-6",

language = "English",

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T2 - Measurement by laser tweezers force spectroscopy

AU - Wang, Shi Kang

AU - Chiu, Jeng Jiann

AU - Lee, Ming Rou

AU - Chou, Shih Chin

AU - Chen, Li Jing

AU - Hwang, Ned H.C.

N1 - Funding Information: Acknowledgements This work was supported by Grants MM-PP-09-02 which is a part of a parallel grant funded to both the Medical Engineering Division, NHRI and the Department of Bioengineering, UCSD (PI: Prof. Y. C. Fung) from the National Health Research Institutes (NHRI), Taiwan, R.O.C. Prof. SK Wang, was a visiting investigator on leave from the Tianjin University, Tianjin, P.R. China.

PY - 2006/9/1

Y1 - 2006/9/1

N2 - Leukocyte adhesion to vascular endothelium is an initial step of many inflammatory diseases. Although the atomic force microscopy (AFM) measurements of leukocyte-endothelial interaction have been recently introduced. with cell adhesion force unbinding curves (CAFUC). We obtained pico-Newton force in the initial interaction between a single living THP-1 cell and HUVEC monolayer using a custom-built laser tweezers (LT) system. The measured quantities included the non-linear force-distance relationship, and the effect of yielding in cell detachment. It is possible to introduce a time scale into the LT cell-detachment experiments for further exploration and more detailed information on the viscoelastic properties of living cells.

AB - Leukocyte adhesion to vascular endothelium is an initial step of many inflammatory diseases. Although the atomic force microscopy (AFM) measurements of leukocyte-endothelial interaction have been recently introduced. with cell adhesion force unbinding curves (CAFUC). We obtained pico-Newton force in the initial interaction between a single living THP-1 cell and HUVEC monolayer using a custom-built laser tweezers (LT) system. The measured quantities included the non-linear force-distance relationship, and the effect of yielding in cell detachment. It is possible to introduce a time scale into the LT cell-detachment experiments for further exploration and more detailed information on the viscoelastic properties of living cells.

KW - Cell adhesion

KW - Endothelial cell

KW - Force unbinding curve

KW - Laser tweezers

KW - Leukocyte

KW - Optical trap

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Leukocyte-endothelium interaction: Measurement by laser tweezers force spectroscopy

Abstract

Keywords

ASJC Scopus subject areas

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