LB1144 Quantitative analysis of differentially expressed proteins in papulopustular rosacea

Papulopustular rosacea (PPR) is a chronic inflammatory skin disease which manifests as red, inflammatory papules or pustules predominantly on the facial region. Although the pathogenesis of PPR is not well understood, an over-reactive inflammatory response plays a role, resulting in altered vasculature and skin sensitivity. Although previous studies have assessed RNA expression or immunohistochemistry staining in tissue, to our knowledge this is the first-time quantitative protein analysis of PPR tissue has been performed. After IRB approval and informed consent, PPR patients (n=4) received biopsies of PPR and non-lesional skin. Biopsies were cultured for 24 hours and the conditioned culture medium subjected to two multiplex proteomic methods (OLINK® and MSD®) to quantify over 100 inflammatory proteins. We identified 21 proteins with statistically significant upregulation in PPR (p