Label-free structural characterization of mitomycin C-modulated wound healing after photorefractive keratectomy by the use of multiphoton microscopy

Wen Lo; Tsung Jen Wang; Wei Liang Chen; Chiu Mei Hsueh; Shean Jen Chen; Yang Fang Chen; Hsiu Chu Chou; Pi Jung Lin; Fung Rong Hu; Chen Yuan Dong

doi:10.1117/1.3432718

Label-free structural characterization of mitomycin C-modulated wound healing after photorefractive keratectomy by the use of multiphoton microscopy

Wen Lo, Tsung Jen Wang, Wei Liang Chen, Chiu Mei Hsueh, Shean Jen Chen, Yang Fang Chen, Hsiu Chu Chou, Pi Jung Lin, Fung Rong Hu, Chen Yuan Dong

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

We applied multiphoton autofluorescence (MAF) and second-harmonic generation (SHG) microscopy to monitor corneal wound healing after photorefractive keratectomy (PRK). Our results show that keratocyte activation can be observed by an increase in its MAF, while SHG imaging of corneal stroma can show the depletion of Bowman's layer after PRK and the reticular collagen deposition in the wound healing stage. Furthermore, quantification of the keratocyte activation and collagen deposition in conjunction with immunohistochemistry and histological images demonstrate the effectiveness of mitomycin C (MMC) in suppressing myofibroblast proliferation and collagen regeneration in the post-PRK wound healing process.

Original language	English
Article number	036005
Journal	Journal of Biomedical Optics
Volume	15
Issue number	3
DOIs	https://doi.org/10.1117/1.3432718
Publication status	Published - May 2010

Keywords

Collagen
Keratocyte activation
Multiphoton autofluorescence excitation
Photorefractive keratectomy
Secondharmonic generation

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Atomic and Molecular Physics, and Optics
Biomedical Engineering
Biomaterials

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10.1117/1.3432718

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title = "Label-free structural characterization of mitomycin C-modulated wound healing after photorefractive keratectomy by the use of multiphoton microscopy",

abstract = "We applied multiphoton autofluorescence (MAF) and second-harmonic generation (SHG) microscopy to monitor corneal wound healing after photorefractive keratectomy (PRK). Our results show that keratocyte activation can be observed by an increase in its MAF, while SHG imaging of corneal stroma can show the depletion of Bowman's layer after PRK and the reticular collagen deposition in the wound healing stage. Furthermore, quantification of the keratocyte activation and collagen deposition in conjunction with immunohistochemistry and histological images demonstrate the effectiveness of mitomycin C (MMC) in suppressing myofibroblast proliferation and collagen regeneration in the post-PRK wound healing process.",

keywords = "Collagen, Keratocyte activation, Multiphoton autofluorescence excitation, Photorefractive keratectomy, Secondharmonic generation",

author = "Wen Lo and Wang, {Tsung Jen} and Chen, {Wei Liang} and Hsueh, {Chiu Mei} and Chen, {Shean Jen} and Chen, {Yang Fang} and Chou, {Hsiu Chu} and Lin, {Pi Jung} and Hu, {Fung Rong} and Dong, {Chen Yuan}",

note = "Funding Information: We would like to acknowledge the support of the National Science Council of Taiwan, National Taiwan University Medical College, and Taipei Medical University Hospital Research Grant No. 97TMU-TMUH-10. We also acknowledge the Universal Foundation of Medical Service and Health Care Education, Universal Eye Center, and Mr. Chen-Chih Yao for assisting with the experiment.",

year = "2010",

month = may,

doi = "10.1117/1.3432718",

language = "English",

volume = "15",

journal = "Journal of Biomedical Optics",

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T1 - Label-free structural characterization of mitomycin C-modulated wound healing after photorefractive keratectomy by the use of multiphoton microscopy

AU - Lo, Wen

AU - Wang, Tsung Jen

AU - Chen, Wei Liang

AU - Hsueh, Chiu Mei

AU - Chen, Shean Jen

AU - Chen, Yang Fang

AU - Chou, Hsiu Chu

AU - Lin, Pi Jung

AU - Hu, Fung Rong

AU - Dong, Chen Yuan

N1 - Funding Information: We would like to acknowledge the support of the National Science Council of Taiwan, National Taiwan University Medical College, and Taipei Medical University Hospital Research Grant No. 97TMU-TMUH-10. We also acknowledge the Universal Foundation of Medical Service and Health Care Education, Universal Eye Center, and Mr. Chen-Chih Yao for assisting with the experiment.

PY - 2010/5

Y1 - 2010/5

N2 - We applied multiphoton autofluorescence (MAF) and second-harmonic generation (SHG) microscopy to monitor corneal wound healing after photorefractive keratectomy (PRK). Our results show that keratocyte activation can be observed by an increase in its MAF, while SHG imaging of corneal stroma can show the depletion of Bowman's layer after PRK and the reticular collagen deposition in the wound healing stage. Furthermore, quantification of the keratocyte activation and collagen deposition in conjunction with immunohistochemistry and histological images demonstrate the effectiveness of mitomycin C (MMC) in suppressing myofibroblast proliferation and collagen regeneration in the post-PRK wound healing process.

AB - We applied multiphoton autofluorescence (MAF) and second-harmonic generation (SHG) microscopy to monitor corneal wound healing after photorefractive keratectomy (PRK). Our results show that keratocyte activation can be observed by an increase in its MAF, while SHG imaging of corneal stroma can show the depletion of Bowman's layer after PRK and the reticular collagen deposition in the wound healing stage. Furthermore, quantification of the keratocyte activation and collagen deposition in conjunction with immunohistochemistry and histological images demonstrate the effectiveness of mitomycin C (MMC) in suppressing myofibroblast proliferation and collagen regeneration in the post-PRK wound healing process.

KW - Collagen

KW - Keratocyte activation

KW - Multiphoton autofluorescence excitation

KW - Photorefractive keratectomy

KW - Secondharmonic generation

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Label-free structural characterization of mitomycin C-modulated wound healing after photorefractive keratectomy by the use of multiphoton microscopy

Abstract

Keywords

ASJC Scopus subject areas

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