Kinetic and crystallographic studies of a redesigned manganese-binding site in cytochrome c peroxidase

Thomas D. Pfister, Amir Y. Mirarefi, Alan J. Gengenbach, Xuan Zhao, Connor Danstrom, Nicole Conatser, Yi Gui Gao, Howard Robinson, Charles F. Zukoski, Andrew H.J. Wang, Yi Lu

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20 Citations (Scopus)


Manganese peroxidase (MnP) from the white rot fungus Phanerochaete chrysosporium contains a manganese-binding site that plays a critical role in its function. Previously, a MnII-binding site was designed into cytochrome c peroxidase (CcP) based on sequence homology (Yeung et al. in Chem. Biol. 4:215-222, 1997; Gengenbach et al. in Biochemistry 38:11425-11432, 1999). Here, we report a redesign of this site based on X-ray structural comparison of MnP and CcP. The variant, CcP(D37E, V45E, H181E), displays 2.5-fold higher catalytic efficiency (kcat/KM) than the variant in the original design, mostly due to a stronger KM of 1.9 mM (vs. 4.1 mM). High-resolution X-ray crystal structures of a metal-free form and a form with CoII at the designed MnII site were also obtained. The metal ion in the engineered metal-binding site overlays well with Mn II bound in MnP, suggesting that this variant is the closest structural model of the MnII-binding site in MnP for which a crystal structure exists. A major difference arises in the distances of the ligands to the metal; the metal-ligand interactions in the CcP variant are much weaker than the corresponding interactions in MnP, probably owing to partial occupancy of metal ion at the designed site, difference in the identity of metal ions (CoII rather than MnII) and other interactions in the second coordination sphere. These results indicate that the metal ion, the ligands, and the environment around the metal-binding site play important roles in tuning the structure and function of metalloenzymes.

Original languageEnglish
Pages (from-to)126-137
Number of pages12
JournalJournal of Biological Inorganic Chemistry
Issue number1
Publication statusPublished - Jan 2007
Externally publishedYes


  • Biomimetic
  • Manganese oxidation
  • Metal-binding site
  • Metalloprotein
  • Protein engineering

ASJC Scopus subject areas

  • Biochemistry
  • Inorganic Chemistry


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