Abstract
Experimentation with a physiomimic system and kinetic analysis exhibited four distinct reaction phases in LDL glycation despite of the type of inducer: glucose or glyoxal. LDL glycation was more sensitive to a status of hyperglycemia (such as 400 mg glucose/100 mL) as evidenced by the reaction order of 0.53. Glucose reacted intensively in the Initial Phase (reaction period 0-2 h) which was identified to result from a parallel mechanism involving both the direct Schiff's product formation and the auto-oxidative cleavages. In contrast, a physiological level of glyoxal revealed merely a reaction order of only 0.09, implicitly indicating a far less sensitive glycation which can be attributed to a mechanism proceeding simply through a molecular Schiff's reaction. On treatment with Psidium guajava L. aqueous extract (PE) (0.01-0.625 mg/mL), a rather unique and significant inhibitory characteristic on LDL glycation was observed with a dose-dependent manner. We attributed such an effect of PE to its distinct abundance of polyphenolic content (165.61 ± 10.39 mg gallic acid equivalent (GAE)/g). Conclusively, PE is an excellent anti-LDL glycative agent whose potential therapeutic uses can be extended to the prevention of a variety of cardiovascular and neurodegenerative diseases associated with glycations.
Original language | English |
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Pages (from-to) | 92-100 |
Number of pages | 9 |
Journal | BioSystems |
Volume | 88 |
Issue number | 1-2 |
DOIs | |
Publication status | Published - Mar 2007 |
Keywords
- Glucose
- Glyoxal
- Kinetic analysis
- LDL glycation
- Psidium guajava L.
ASJC Scopus subject areas
- Statistics and Probability
- Modelling and Simulation
- General Biochemistry,Genetics and Molecular Biology
- Applied Mathematics