Isolation and Structure Elucidation of Secondary Metabolites From an Endophytic Fungus Annulohypoxylon ilanense

Ming Jen Cheng; Shuen Shin Yang; Ming Der Wu; Hsun Hsuo Chang; Yueh Hsiung Kuo; Sung Yuan Hsieh; Jih Jung Chen; Ho Cheng Wu

doi:10.1177/1934578X19857906

Isolation and Structure Elucidation of Secondary Metabolites From an Endophytic Fungus Annulohypoxylon ilanense

Ming Jen Cheng, Shuen Shin Yang, Ming Der Wu, Hsun Hsuo Chang, Yueh Hsiung Kuo, Sung Yuan Hsieh, Jih Jung Chen, Ho Cheng Wu

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

Fractionation of the EtOAc-soluble fraction of the liquid fermentation of an endophytic fungus Annulohypoxylon ilanense associated with the wood of medicinal plant Cinnamomum species resulted in the isolation of 1 new α-pyrone, ilanpyrone (1), along with 3 previously identified compounds, 2 to 4. Their structures were elucidated by means of spectroscopic and mass-spectrometric analyses, especially 1D and 2D nuclear magnetic resonance (NMR) spectroscopy as well as high-resolution electrospray ionization mass spectrometry. The isolated compounds were tested for their cytotoxicity against MCF-7, NCI-H460, and SF-268 cell lines using the MTT assay. Among them, compounds 1 and 4 were found to have moderate-to-weak cytotoxic effects against these 3 cell lines in vitro.

Original language	English
Journal	Natural Product Communications
Volume	14
Issue number	9
DOIs	https://doi.org/10.1177/1934578X19857906
Publication status	Published - 2019
Externally published	Yes

Keywords

Annulohypoxylon ilanense
cytotoxicity
xylariaceae
α-Pyrone

ASJC Scopus subject areas

Pharmacology
Drug Discovery
Plant Science
Complementary and alternative medicine

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10.1177/1934578X19857906

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title = "Isolation and Structure Elucidation of Secondary Metabolites From an Endophytic Fungus Annulohypoxylon ilanense",

abstract = "Fractionation of the EtOAc-soluble fraction of the liquid fermentation of an endophytic fungus Annulohypoxylon ilanense associated with the wood of medicinal plant Cinnamomum species resulted in the isolation of 1 new α-pyrone, ilanpyrone (1), along with 3 previously identified compounds, 2 to 4. Their structures were elucidated by means of spectroscopic and mass-spectrometric analyses, especially 1D and 2D nuclear magnetic resonance (NMR) spectroscopy as well as high-resolution electrospray ionization mass spectrometry. The isolated compounds were tested for their cytotoxicity against MCF-7, NCI-H460, and SF-268 cell lines using the MTT assay. Among them, compounds 1 and 4 were found to have moderate-to-weak cytotoxic effects against these 3 cell lines in vitro.",

keywords = "Annulohypoxylon ilanense, cytotoxicity, xylariaceae, α-Pyrone",

author = "Cheng, {Ming Jen} and Yang, {Shuen Shin} and Wu, {Ming Der} and Chang, {Hsun Hsuo} and Kuo, {Yueh Hsiung} and Hsieh, {Sung Yuan} and Chen, {Jih Jung} and Wu, {Ho Cheng}",

note = "Funding Information: The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This work was kindly supported by the Food Industry Research and Development Institute (FIRDI) and partially supported by Ministry of Science and Techology, R.O.C. (MOST-106-2320-B-037-014). Publisher Copyright: {\textcopyright} The Author(s) 2019.",

year = "2019",

doi = "10.1177/1934578X19857906",

language = "English",

volume = "14",

journal = "Natural Product Communications",

issn = "1934-578X",

publisher = "SAGE Publications Inc.",

number = "9",

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T1 - Isolation and Structure Elucidation of Secondary Metabolites From an Endophytic Fungus Annulohypoxylon ilanense

AU - Cheng, Ming Jen

AU - Yang, Shuen Shin

AU - Wu, Ming Der

AU - Chang, Hsun Hsuo

AU - Kuo, Yueh Hsiung

AU - Hsieh, Sung Yuan

AU - Chen, Jih Jung

AU - Wu, Ho Cheng

N1 - Funding Information: The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This work was kindly supported by the Food Industry Research and Development Institute (FIRDI) and partially supported by Ministry of Science and Techology, R.O.C. (MOST-106-2320-B-037-014). Publisher Copyright: © The Author(s) 2019.

PY - 2019

Y1 - 2019

N2 - Fractionation of the EtOAc-soluble fraction of the liquid fermentation of an endophytic fungus Annulohypoxylon ilanense associated with the wood of medicinal plant Cinnamomum species resulted in the isolation of 1 new α-pyrone, ilanpyrone (1), along with 3 previously identified compounds, 2 to 4. Their structures were elucidated by means of spectroscopic and mass-spectrometric analyses, especially 1D and 2D nuclear magnetic resonance (NMR) spectroscopy as well as high-resolution electrospray ionization mass spectrometry. The isolated compounds were tested for their cytotoxicity against MCF-7, NCI-H460, and SF-268 cell lines using the MTT assay. Among them, compounds 1 and 4 were found to have moderate-to-weak cytotoxic effects against these 3 cell lines in vitro.

AB - Fractionation of the EtOAc-soluble fraction of the liquid fermentation of an endophytic fungus Annulohypoxylon ilanense associated with the wood of medicinal plant Cinnamomum species resulted in the isolation of 1 new α-pyrone, ilanpyrone (1), along with 3 previously identified compounds, 2 to 4. Their structures were elucidated by means of spectroscopic and mass-spectrometric analyses, especially 1D and 2D nuclear magnetic resonance (NMR) spectroscopy as well as high-resolution electrospray ionization mass spectrometry. The isolated compounds were tested for their cytotoxicity against MCF-7, NCI-H460, and SF-268 cell lines using the MTT assay. Among them, compounds 1 and 4 were found to have moderate-to-weak cytotoxic effects against these 3 cell lines in vitro.

KW - Annulohypoxylon ilanense

KW - cytotoxicity

KW - xylariaceae

KW - α-Pyrone

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Isolation and Structure Elucidation of Secondary Metabolites From an Endophytic Fungus Annulohypoxylon ilanense

Abstract

Keywords

ASJC Scopus subject areas

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