Isolation and immunomodulatory effect of homoisoflavones and flavones from Agave sisalana Perrine Ex Engelm

Pi Yu Chen; Yuh Chi Kuo; Chin Hui Chen; Yueh Hsiung Kuo; Ching Kuo Lee

doi:10.3390/molecules14051789

Isolation and immunomodulatory effect of homoisoflavones and flavones from Agave sisalana Perrine Ex Engelm

Pi Yu Chen, Yuh Chi Kuo, Chin Hui Chen, Yueh Hsiung Kuo, Ching Kuo Lee

Research output: Contribution to journal › Article › peer-review

44 Citations (Scopus)

Abstract

Three known flavones and seven known homoisoflavonoids were isolated from the methanolic extract of the leaves of Agave sisalana Perrine ex Engelm. Their structures were elucidated on the basis of spectroscopic analysis. The isolated compounds were also evaluated for immunopharmacological activity. PBMC were used as target cells, and cell proliferation was determined by ³H- thymidine uptake. (±)-3,9-Dihydroeucomin (4), dihydrobonducellin (5), and 5,7-dihydroxy-3-(4′-hydroxybenzyl)-4-chromanone (7) showed inhibitory effects on PBMC proliferation activated by PHA with IC₅₀ values 19.4, 73.8, and 58.8 μM, respectively. All three compounds significantly inhibited the production of IL-2 and IFN-γ in activated PBMC in a concentration-dependent manner.

Original language	English
Pages (from-to)	1789-1795
Number of pages	7
Journal	Molecules
Volume	14
Issue number	5
DOIs	https://doi.org/10.3390/molecules14051789
Publication status	Published - May 2009

Keywords

Agave sisalana Perrine ex Engelm
Homoisoflavonoid
IFN-γ
IL-2
PBMC

ASJC Scopus subject areas

Analytical Chemistry
Chemistry (miscellaneous)
Molecular Medicine
Pharmaceutical Science
Drug Discovery
Physical and Theoretical Chemistry
Organic Chemistry

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10.3390/molecules14051789

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title = "Isolation and immunomodulatory effect of homoisoflavones and flavones from Agave sisalana Perrine Ex Engelm",

abstract = "Three known flavones and seven known homoisoflavonoids were isolated from the methanolic extract of the leaves of Agave sisalana Perrine ex Engelm. Their structures were elucidated on the basis of spectroscopic analysis. The isolated compounds were also evaluated for immunopharmacological activity. PBMC were used as target cells, and cell proliferation was determined by 3H- thymidine uptake. (±)-3,9-Dihydroeucomin (4), dihydrobonducellin (5), and 5,7-dihydroxy-3-(4′-hydroxybenzyl)-4-chromanone (7) showed inhibitory effects on PBMC proliferation activated by PHA with IC50 values 19.4, 73.8, and 58.8 μM, respectively. All three compounds significantly inhibited the production of IL-2 and IFN-γ in activated PBMC in a concentration-dependent manner.",

keywords = "Agave sisalana Perrine ex Engelm, Homoisoflavonoid, IFN-γ, IL-2, PBMC",

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AU - Chen, Chin Hui

AU - Kuo, Yueh Hsiung

AU - Lee, Ching Kuo

PY - 2009/5

Y1 - 2009/5

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KW - IFN-γ

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Isolation and immunomodulatory effect of homoisoflavones and flavones from Agave sisalana Perrine Ex Engelm

Abstract

Keywords

ASJC Scopus subject areas

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