Involvement of phosphatidylcholine-phospholipase C and protein kinase C in peptidoglycan-induced nuclear factor-κB activation and cyclooxygenase-2 expression in RAW 264.7 macrophages

Jam Inn Tzeng; Bing Chang Chen; Huey Mei Chang; Jhi Joung Wang; Munisamy Sureshbabu; Ming Hsien Chien; Ming Jen Hsu; Mauo Ying Bien; Wen Ta Chiu; Chuang Ye Hong; Chien Huang Lin

doi:10.1016/j.phrs.2009.09.005

Involvement of phosphatidylcholine-phospholipase C and protein kinase C in peptidoglycan-induced nuclear factor-κB activation and cyclooxygenase-2 expression in RAW 264.7 macrophages

Jam Inn Tzeng, Bing Chang Chen, Huey Mei Chang, Jhi Joung Wang, Munisamy Sureshbabu, Ming Hsien Chien, Ming Jen Hsu, Mauo Ying Bien, Wen Ta Chiu, Chuang Ye Hong, Chien Huang Lin

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

In this study, we examined the role of phosphatidylcholine-phospholipase C (PC-PLC) and protein kinase C (PKC) in peptidoglycan (PGN)-induced nuclear factor-κB (NF-κB) activation and cyclooxygenase-2 (COX-2) expression in RAW 264.7 macrophages. PGN-induced COX-2 expression was attenuated by a PC-PLC inhibitor (D609) and by PKC inhibitors (Go 6976 and Ro 31-8220), but not by a phosphatidylinositol-PLC (PI-PLC) inhibitor (U-73122). PGN caused an increase in PKC activity, and this effect was inhibited by D609, Go 6976, and Ro 31-8220, but not by U-73122. Furthermore, the PGN-mediated increases in κB-luciferase activity were also inhibited by D609 and Ro 31-8220. Our data demonstrate that PGN activates PC-PLC which induces PKC activation; this in turn initiates NF-κB activation, and ultimately induces COX-2 expression in RAW 264.7 macrophages.

Original language	English
Pages (from-to)	162-166
Number of pages	5
Journal	Pharmacological Research
Volume	61
Issue number	2
DOIs	https://doi.org/10.1016/j.phrs.2009.09.005
Publication status	Published - Feb 2010

Keywords

COX-2
Inflammation
NF-κB
Peptidoglycan
Protein kinase C

ASJC Scopus subject areas

Pharmacology

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10.1016/j.phrs.2009.09.005

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Tzeng, J. I., Chen, B. C., Chang, H. M., Wang, J. J., Sureshbabu, M., Chien, M. H., Hsu, M. J., Bien, M. Y., Chiu, W. T., Hong, C. Y., & Lin, C. H. (2010). Involvement of phosphatidylcholine-phospholipase C and protein kinase C in peptidoglycan-induced nuclear factor-κB activation and cyclooxygenase-2 expression in RAW 264.7 macrophages. Pharmacological Research, 61(2), 162-166. https://doi.org/10.1016/j.phrs.2009.09.005

Involvement of phosphatidylcholine-phospholipase C and protein kinase C in peptidoglycan-induced nuclear factor-κB activation and cyclooxygenase-2 expression in RAW 264.7 macrophages. / Tzeng, Jam Inn; Chen, Bing Chang; Chang, Huey Mei et al.
In: Pharmacological Research, Vol. 61, No. 2, 02.2010, p. 162-166.

Research output: Contribution to journal › Article › peer-review

Tzeng, JI, Chen, BC, Chang, HM, Wang, JJ, Sureshbabu, M, Chien, MH , Hsu, MJ, Bien, MY, Chiu, WT , Hong, CY & Lin, CH 2010, 'Involvement of phosphatidylcholine-phospholipase C and protein kinase C in peptidoglycan-induced nuclear factor-κB activation and cyclooxygenase-2 expression in RAW 264.7 macrophages', Pharmacological Research, vol. 61, no. 2, pp. 162-166. https://doi.org/10.1016/j.phrs.2009.09.005

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abstract = "In this study, we examined the role of phosphatidylcholine-phospholipase C (PC-PLC) and protein kinase C (PKC) in peptidoglycan (PGN)-induced nuclear factor-κB (NF-κB) activation and cyclooxygenase-2 (COX-2) expression in RAW 264.7 macrophages. PGN-induced COX-2 expression was attenuated by a PC-PLC inhibitor (D609) and by PKC inhibitors (Go 6976 and Ro 31-8220), but not by a phosphatidylinositol-PLC (PI-PLC) inhibitor (U-73122). PGN caused an increase in PKC activity, and this effect was inhibited by D609, Go 6976, and Ro 31-8220, but not by U-73122. Furthermore, the PGN-mediated increases in κB-luciferase activity were also inhibited by D609 and Ro 31-8220. Our data demonstrate that PGN activates PC-PLC which induces PKC activation; this in turn initiates NF-κB activation, and ultimately induces COX-2 expression in RAW 264.7 macrophages.",

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AU - Hsu, Ming Jen

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AU - Chiu, Wen Ta

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Involvement of phosphatidylcholine-phospholipase C and protein kinase C in peptidoglycan-induced nuclear factor-κB activation and cyclooxygenase-2 expression in RAW 264.7 macrophages

Abstract

Keywords

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