Involvement of cholesterol in Campylobacter jejuni cytolethal distending toxin-induced pathogenesis

Cheng Kuo Lai, Jing Chi Su, Yu Hsin Lin, Chia Shuo Chang, Chun Lung Feng, Hwai Jeng Lin, Chun Jung Lin, Guan Chin Tseng, Hsin Ho Liu, Jer Tsong Hsieh, Hong Lin Su, Chih Ho Lai

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)


Aim: The aim of this study was to investigate whether cholesterol plays a pivotal role in cytolethal distending toxin (CDT) mediated pathogenic effects in hosts. Materials & methods: The molecular mechanisms underlying cholesterol sequestering conferred resistance to CDT-induced DNA double-strand breaks (DSBs) and cell cycle arrest were investigated. Histopathological analysis was conducted for evaluating CDT-induced intestinal inflammation in mouse. Results: CDT actions were attenuated by treatment of cells with methyl-β-cyclodextrin (MβCD). Severe intestinal inflammation induced by CDT treatment was observed in high-cholesterol diet-fed mice, but not in normal diet-fed mice, indicating that cholesterol is essential for CDT intoxication. Conclusion: Our findings demonstrate a molecular link between Campylobacter jejuni CDT and cholesterol, which is crucial to facilitate CDT-induced pathogenesis in hosts.

Original languageEnglish
Pages (from-to)489-501
Number of pages13
JournalFuture Microbiology
Issue number4
Publication statusPublished - Apr 1 2015


  • Campylobacter jejuni
  • DNA double-strand breaks
  • cell cycle
  • cholesterol
  • cytolethal distending toxin

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)


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