Investigation on liquid chromatographic separation of basic compounds using silica column with aqueous/organic mobile phase containing triethylamine and acetic acid

An-Bang Wu, Ming Chun Huang, Hsiu O. Ho, Geng Cheng Yeh, Ming Thau Sheu

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

A high-performance liquid chromatography (HPLC) method using silica column eluted with aqueous solvent mobile phase containing triethylamine (TEA) and acetic acid (ACH) at trace percentages was characterized for the analysis of basic compounds. The key mechanism of this system is ion-exchange accompanying interaction of silanol groups. The increase in the ACH concentration in the mobile phase minimizes the ionization of the silanol group, leading to reduced retention time. However, the greater extent of ionization of silanol caused by the increase of TEA concentration helps to retain basic compounds in the column. Further, the protonated TEA that is positively charged also competes for the ionized silanol group with basic compounds, resulting in the modification of retention time. On the other hand, the retention becomes longer with increasing proportion of either organic or aqueous solvent in mobile phase, and partial replacement of methanol with acetonitrile.

Original languageEnglish
Pages (from-to)443-449
Number of pages7
JournalBiomedical Chromatography
Volume18
Issue number7
DOIs
Publication statusPublished - Sept 2004

Keywords

  • Acetic acid
  • Aqueous eluents
  • Basic compounds
  • Silica column
  • TH, tetrahydrozoline HC1
  • Triethylamine

ASJC Scopus subject areas

  • Drug Discovery
  • Analytical Chemistry
  • Molecular Biology
  • Biochemistry
  • Clinical Biochemistry
  • Pharmacology

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