Intrauterine growth restriction alters kidney metabolism at the end of nephrogenesis

Sheng Yuan Ho, Merryl Esther Yuliana, Hsiu Chu Chou, Chung Ming Chen

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Background: This study investigated the effect of uteroplacental insufficiency (UPI) on renal development by detecting metabolic alterations in the kidneys of rats with intrauterine growth restriction (IUGR). Methods: On gestational day 17, pregnant Sprague Dawley rats were selected and allocated randomly to either the IUGR group or the control group. The IUGR group received a protocol involving the closure of bilateral uterine vessels, while the control group underwent a sham surgery. The rat pups were delivered on gestational day 22 by natural means. Pups were randomly recruited from both the control and IUGR groups on the seventh day after birth. The kidneys were surgically removed to conduct Western blot and metabolomic analyses. Results: IUGR was produced by UPI, as evidenced by the significantly lower body weights of the pups with IUGR compared to the control pups on postnatal day 7. UPI significantly increased the levels of cleaved caspase-3 (p < 0.05) and BAX/Bcl-2 (p < 0.01) in the pups with IUGR. Ten metabolites exhibited statistically significant differences between the groups (q < 0.05). Metabolic pathway enrichment analysis demonstrated statistically significant variations between the groups in the metabolism related to fructose and mannose, amino and nucleotide sugars, and inositol phosphate. Conclusions: UPI alters kidney metabolism in growth-restricted newborn rats and induces renal apoptosis. The results of our study have the potential to provide new insights into biomarkers and metabolic pathways that are involved in the kidney changes generated by IUGR.

Original languageEnglish
Article number50
JournalNutrition and Metabolism
Volume20
Issue number1
DOIs
Publication statusPublished - Dec 2023

Keywords

  • Intrauterine growth restriction
  • Kidney
  • Metabolomics
  • Nephrogenesis
  • Uteroplacental insufficiency

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Medicine (miscellaneous)
  • Nutrition and Dietetics

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