TY - JOUR
T1 - Intraarticular injection for rabbit knee osteoarthritis
T2 - effectiveness among hyaluronic acid, platelet-rich plasma, and mesenchymal stem cells
AU - Chen, Yu Chun
AU - Hsu, Yuan Ming
AU - Tan, Kinn Poay
AU - Fang, Hsu Wei
AU - Chang, Chih Hung
N1 - Funding Information:
The authors would like to thank: Yu-Ting Bai and Alfred Lin for guidance in the statistical analyses; Far Eastern Memorial Hospital for their financial support (project number: FEMH-2012-SCRM-A-003; FEMH-2014-C-025; FEMH-2014-SCRM-005); Far Eastern Memorial Hospital Core Laboratory I & II for providing facilities and instruments; and Far Eastern Memorial Hospital Laboratory Animal Center for assisting with the animal care and management.
Publisher Copyright:
© 2018 Taiwan Institute of Chemical Engineers
Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.
PY - 2018/10/1
Y1 - 2018/10/1
N2 - Both hyaluronic acid (HA) and platelet-rich plasma (PRP) injection are the common prescribed treatments for osteoarthritis (OA) patients. HA can serve as a good lubricant to reduce the fraction force of cartilage surface while PRP provide several growth factors to assist tissue repair. Considering the efficiency of tissue regeneration, mesenchymal stem cells (MSCs) injections becomes an attractive method because of their self-renewal and multi-differentiation potential. Thus, in the study, we compared the treatment effect of PRP and HA with/without MSCs. Eighteen rabbits underwent anterior cruciate ligament transection for OA knee induction, and then injected with saline, PRP, HA, HA+PRP, PRP+MSCs or HA+MSCs intraarticularly for treatment effect evaluation. Compared with the results of HA group, the cartilage treated with HA+PRP and PRP+MSCs showed better repair results in micromorphology and glycosaminoglycan synthesis. We also found that the Osteoarthritis Research Society International (OASRI) scores in HA+PRP and PRP+MSC groups were significant lower than that of the others at month 1 and month 3, separately. This indicates that the combination of HA and PRP could provide short-term tissue protection effect while PRP and MSC could provide a long-term tissue regeneration effect. PRP+MSC could be considered as a promising biological method for OA treatment.
AB - Both hyaluronic acid (HA) and platelet-rich plasma (PRP) injection are the common prescribed treatments for osteoarthritis (OA) patients. HA can serve as a good lubricant to reduce the fraction force of cartilage surface while PRP provide several growth factors to assist tissue repair. Considering the efficiency of tissue regeneration, mesenchymal stem cells (MSCs) injections becomes an attractive method because of their self-renewal and multi-differentiation potential. Thus, in the study, we compared the treatment effect of PRP and HA with/without MSCs. Eighteen rabbits underwent anterior cruciate ligament transection for OA knee induction, and then injected with saline, PRP, HA, HA+PRP, PRP+MSCs or HA+MSCs intraarticularly for treatment effect evaluation. Compared with the results of HA group, the cartilage treated with HA+PRP and PRP+MSCs showed better repair results in micromorphology and glycosaminoglycan synthesis. We also found that the Osteoarthritis Research Society International (OASRI) scores in HA+PRP and PRP+MSC groups were significant lower than that of the others at month 1 and month 3, separately. This indicates that the combination of HA and PRP could provide short-term tissue protection effect while PRP and MSC could provide a long-term tissue regeneration effect. PRP+MSC could be considered as a promising biological method for OA treatment.
KW - Cell therapy
KW - Hyaluronic acid
KW - Mesenchymal stem cells
KW - Osteoarthritis
KW - Platelet-rich plasma
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U2 - 10.1016/j.jtice.2018.05.051
DO - 10.1016/j.jtice.2018.05.051
M3 - Article
AN - SCOPUS:85049571316
SN - 1876-1070
VL - 91
SP - 138
EP - 145
JO - Journal of the Taiwan Institute of Chemical Engineers
JF - Journal of the Taiwan Institute of Chemical Engineers
ER -