Intestinal microbiome in children with severe and complicated acute viral gastroenteritis

Shih Yen Chen, Chi Neu Tsai, Yun Shien Lee, Chun Yuan Lin, Kuan Yeh Huang, Hsun Ching Chao, Ming Wei Lai, Cheng Hsun Chiu

Research output: Contribution to journalArticlepeer-review

52 Citations (Scopus)

Abstract

The aim of the present study was to evaluate the microbiota of children with severe or complicated acute viral gastroenteritis (AGE). To that end, next-generation sequencing (NGS) technology was used to sequence the 16S ribosomal RNA (16S rRNA) gene in 20 hospitalized pediatric patients with severe or complicated AGE and a further 20 otherwise healthy children; the fecal microbiome was then assessed. Comparative metagenomics data were analyzed by a Wilcoxon rank-sum test and hierarchical clustering analysis of bacterial reads. The statistical analyses showed a significantly decreased Shannon diversity index (entropy score) of the intestinal microbiota in patients with severe AGE compared with normal controls (P = 0.017) and patients with mild-to-moderate AGE (P = 0.011). The intestinal microbiota score of the 5 patients with rotavirus AGE was significantly lower than that of those with norovirus infection (P = 0.048). Greater richness in Campylobacteraceae (P = 0.0003), Neisseriaceae (P = 0.0115), Methylobacteriaceae (P = 0.0004), Sphingomonadaceae (P = 0.0221), and Enterobacteriaceae (P = 0.0451) was found in patients with complicated AGE compared with normal controls. The data suggest a significant reduction in intestinal microbial diversity in patients with severe AGE, particularly those with rotavirus infection.

Original languageEnglish
Article number46130
JournalScientific Reports
Volume7
DOIs
Publication statusPublished - Apr 11 2017
Externally publishedYes

ASJC Scopus subject areas

  • General

Fingerprint

Dive into the research topics of 'Intestinal microbiome in children with severe and complicated acute viral gastroenteritis'. Together they form a unique fingerprint.

Cite this