TY - JOUR
T1 - Interleukin-6 Protein Expression Is More Important Than Interleukin-6 mRNA Levels in Assessing Surgical Invasiveness
AU - Huang, Tsung Jen
AU - Li, Yen Yao
AU - Weng, Yi Jan
AU - Cheng, Chin Chang
AU - Hsu, Robert Wen Wei
N1 - Funding Information:
The authors thank the National Science Council, Taiwan, for financially supporting this research under contract no. NSC 92-2314-B-182A-074 (NMRPG2080). They also thank Miss Sharon Lei and Miss Yi-Fang Tsai for their comments and assistance regarding statistical analysis, as well as Ted Knoy for critically editing this paper.
Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2007/9
Y1 - 2007/9
N2 - Background: Interleukin-6 (IL-6) protein has been recognized as a sensitive marker of surgical stress response. However, little is known about the clinical significance of IL-6 mRNA levels as a marker of surgical stress. This study aims to examine the role of IL-6 mRNA expression in comparing the tissue invasiveness of microendoscopic discectomy (MED) and open discectomy (OD). Methods: Twenty-three consecutive patients were randomly selected to undergo either MED or OD. The total RNA was extracted from the peripheral whole blood of patients at pre-op and at 1, 2, 4, 8, 12 h post-op. The real-time reverse transcription polymerase chain reaction (RT-PCR) using the SYBR Green I fluorescence dye and the 2 -ΔΔCt method was adopted to measure the IL-6 gene expression. Results: The quantitative changes of IL-6 mRNA expression in MED and OD patients at different times post-op differed significantly, P = 0.04. Experimental results indicate that the changes in IL-6 mRNA expression in OD and MED groups varied significantly at 1 h, 12 h post-op, 10.26-fold versus 4.42-fold and 52.15fold versus 26.78-fold increase, respectively. Although IL-6 mRNA expression demonstrated an earlier difference than protein levels at 1 h post-op, IL-6 mRNA levels were found to be significantly affected after surgical procedures. Furthermore, compared with our enzyme-linked immunosorbent assay data, no significant correlation existed between IL-6 mRNA and protein levels at any post-op time interval. Conclusions: We conclude that IL-6 mRNA expression using RT-PCR to extract the total RNA from a patient's peripheral whole blood is more sensitive than protein levels but can be significantly affected by surgical procedures. The enzyme-linked immunosorbent assay data on IL-6 protein expression are more consistent and significant than IL-6 mRNA levels in comparing tissue invasiveness between MED and OD.
AB - Background: Interleukin-6 (IL-6) protein has been recognized as a sensitive marker of surgical stress response. However, little is known about the clinical significance of IL-6 mRNA levels as a marker of surgical stress. This study aims to examine the role of IL-6 mRNA expression in comparing the tissue invasiveness of microendoscopic discectomy (MED) and open discectomy (OD). Methods: Twenty-three consecutive patients were randomly selected to undergo either MED or OD. The total RNA was extracted from the peripheral whole blood of patients at pre-op and at 1, 2, 4, 8, 12 h post-op. The real-time reverse transcription polymerase chain reaction (RT-PCR) using the SYBR Green I fluorescence dye and the 2 -ΔΔCt method was adopted to measure the IL-6 gene expression. Results: The quantitative changes of IL-6 mRNA expression in MED and OD patients at different times post-op differed significantly, P = 0.04. Experimental results indicate that the changes in IL-6 mRNA expression in OD and MED groups varied significantly at 1 h, 12 h post-op, 10.26-fold versus 4.42-fold and 52.15fold versus 26.78-fold increase, respectively. Although IL-6 mRNA expression demonstrated an earlier difference than protein levels at 1 h post-op, IL-6 mRNA levels were found to be significantly affected after surgical procedures. Furthermore, compared with our enzyme-linked immunosorbent assay data, no significant correlation existed between IL-6 mRNA and protein levels at any post-op time interval. Conclusions: We conclude that IL-6 mRNA expression using RT-PCR to extract the total RNA from a patient's peripheral whole blood is more sensitive than protein levels but can be significantly affected by surgical procedures. The enzyme-linked immunosorbent assay data on IL-6 protein expression are more consistent and significant than IL-6 mRNA levels in comparing tissue invasiveness between MED and OD.
KW - interleukin-6, mRNA, microendoscopic discectomy (MED)
KW - open discectomy
KW - real-time RT-PCR (reverse transcription polymerase chain reaction)
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U2 - 10.1016/j.jss.2006.09.037
DO - 10.1016/j.jss.2006.09.037
M3 - Article
C2 - 17610900
AN - SCOPUS:34547956113
SN - 0022-4804
VL - 142
SP - 53
EP - 58
JO - Journal of Surgical Research
JF - Journal of Surgical Research
IS - 1
ER -