TY - JOUR
T1 - Interleukin-20 targets renal cells and is associated with chronic kidney disease
AU - Wei, Chi Chen
AU - Li, Hsing Hui
AU - Hsu, Yu Hsiang
AU - Hsing, Chung-Hsi
AU - Sung, Junne Ming
AU - Chang, Ming Shi
N1 - Funding Information:
This study was supported by a Grant from the National Science Council (NSC 96-2628-B-006 -055 -MY3), Taiwan, Republic of China.
Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2008/9/26
Y1 - 2008/9/26
N2 - Interleukin (IL)-10 is an anti-inflammatory factor that suppresses renal fibrosis and improves renal function in CKD rats. IL-20 belongs to the IL-10 family; therefore, we sought to determine whether IL-20 is involved in CKD. CKD patients at stage five expressed significantly higher IL-20 in serum than controls. Immunohistochemical staining demonstrated that more IL-20 protein was expressed in the kidney tubular-epithelial cells, mesangial cells, and immune cells of CKD rats with a 5/6 nephrectomy. The lung, liver, and heart tissue of CKD rats also overexpressed IL-20. Thus, we treated two tubular epithelial cells, TKPTS and M-1 cells, with IL-20 to study its effects on CKD. IL-20 treatment induced apoptosis in these cells via caspase-3 activation. Incubating IL-20 with rat interstitial fibroblasts, NRK-49F cells, upregulated TGF-β1production, one key inducer for renal fibrogenesis. Therefore, IL-20 injured renal epithelial cells and induced fibroblasts to produce TGF-β1 that hastened the progression of CKD.
AB - Interleukin (IL)-10 is an anti-inflammatory factor that suppresses renal fibrosis and improves renal function in CKD rats. IL-20 belongs to the IL-10 family; therefore, we sought to determine whether IL-20 is involved in CKD. CKD patients at stage five expressed significantly higher IL-20 in serum than controls. Immunohistochemical staining demonstrated that more IL-20 protein was expressed in the kidney tubular-epithelial cells, mesangial cells, and immune cells of CKD rats with a 5/6 nephrectomy. The lung, liver, and heart tissue of CKD rats also overexpressed IL-20. Thus, we treated two tubular epithelial cells, TKPTS and M-1 cells, with IL-20 to study its effects on CKD. IL-20 treatment induced apoptosis in these cells via caspase-3 activation. Incubating IL-20 with rat interstitial fibroblasts, NRK-49F cells, upregulated TGF-β1production, one key inducer for renal fibrogenesis. Therefore, IL-20 injured renal epithelial cells and induced fibroblasts to produce TGF-β1 that hastened the progression of CKD.
KW - Apoptosis
KW - Chronic kidney disease
KW - Cytokines
KW - Fibroblasts
KW - Renal epithelial cells
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U2 - 10.1016/j.bbrc.2008.07.039
DO - 10.1016/j.bbrc.2008.07.039
M3 - Article
C2 - 18639518
AN - SCOPUS:48849099770
SN - 0006-291X
VL - 374
SP - 448
EP - 453
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -