Interaction between the left-handed Z-DNA and polyamine The crystal structure of the d(CG)3 and N-(2-aminoethyl)-1,4-diamino-butane complex

Hirofumi Ohishi; Shoko Kunisawa; Gijs van der Marel; Jacques H. van Boom; Alexander Rich; Andrew H.J. Wang; Ken ichi Tomita; Toshio Hakoshima

doi:10.1016/0014-5793(91)80694-X

Interaction between the left-handed Z-DNA and polyamine The crystal structure of the d(CG)₃ and N-(2-aminoethyl)-1,4-diamino-butane complex

Hirofumi Ohishi, Shoko Kunisawa, Gijs van der Marel, Jacques H. van Boom, Alexander Rich, Andrew H.J. Wang, Ken ichi Tomita, Toshio Hakoshima

Research output: Contribution to journal › Article › peer-review

29 Citations (Scopus)

Abstract

The DNA fragment d(CG)₃ was co-crystallized with N-(2-aminoethyl)-1,4-diaminobutane (PA(24)), a chemically synthesized polyamine. The complex crystal contained one polyamine, 3 magnesium cations and one sodium cation per duplex of d(CG)₃, and well diffracted the X-ray intensities up to 1.0 Å resolution. The d(CG)₃ took a left-handed Z-DNA conformation, and the PA(24) molecule electrostatically interacted with the phosphate groups of the d(CG)₃ duplex.

Original language	English
Pages (from-to)	238-244
Number of pages	7
Journal	FEBS Letters
Volume	284
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(91)80694-X
Publication status	Published - Jun 24 1991
Externally published	Yes

Keywords

Crystal structure
Molecular conformation
Molecular interaction
Polyamine
Z-DNA

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

Access to Document

10.1016/0014-5793(91)80694-X

Cite this

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title = "Interaction between the left-handed Z-DNA and polyamine The crystal structure of the d(CG)3 and N-(2-aminoethyl)-1,4-diamino-butane complex",

abstract = "The DNA fragment d(CG)3 was co-crystallized with N-(2-aminoethyl)-1,4-diaminobutane (PA(24)), a chemically synthesized polyamine. The complex crystal contained one polyamine, 3 magnesium cations and one sodium cation per duplex of d(CG)3, and well diffracted the X-ray intensities up to 1.0 {\AA} resolution. The d(CG)3 took a left-handed Z-DNA conformation, and the PA(24) molecule electrostatically interacted with the phosphate groups of the d(CG)3 duplex.",

keywords = "Crystal structure, Molecular conformation, Molecular interaction, Polyamine, Z-DNA",

author = "Hirofumi Ohishi and Shoko Kunisawa and {van der Marel}, Gijs and {van Boom}, {Jacques H.} and Alexander Rich and Wang, {Andrew H.J.} and Tomita, {Ken ichi} and Toshio Hakoshima",

year = "1991",

month = jun,

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doi = "10.1016/0014-5793(91)80694-X",

language = "English",

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journal = "FEBS Letters",

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T1 - Interaction between the left-handed Z-DNA and polyamine The crystal structure of the d(CG)3 and N-(2-aminoethyl)-1,4-diamino-butane complex

AU - Ohishi, Hirofumi

AU - Kunisawa, Shoko

AU - van der Marel, Gijs

AU - van Boom, Jacques H.

AU - Rich, Alexander

AU - Wang, Andrew H.J.

AU - Tomita, Ken ichi

AU - Hakoshima, Toshio

PY - 1991/6/24

Y1 - 1991/6/24

N2 - The DNA fragment d(CG)3 was co-crystallized with N-(2-aminoethyl)-1,4-diaminobutane (PA(24)), a chemically synthesized polyamine. The complex crystal contained one polyamine, 3 magnesium cations and one sodium cation per duplex of d(CG)3, and well diffracted the X-ray intensities up to 1.0 Å resolution. The d(CG)3 took a left-handed Z-DNA conformation, and the PA(24) molecule electrostatically interacted with the phosphate groups of the d(CG)3 duplex.

AB - The DNA fragment d(CG)3 was co-crystallized with N-(2-aminoethyl)-1,4-diaminobutane (PA(24)), a chemically synthesized polyamine. The complex crystal contained one polyamine, 3 magnesium cations and one sodium cation per duplex of d(CG)3, and well diffracted the X-ray intensities up to 1.0 Å resolution. The d(CG)3 took a left-handed Z-DNA conformation, and the PA(24) molecule electrostatically interacted with the phosphate groups of the d(CG)3 duplex.

KW - Crystal structure

KW - Molecular conformation

KW - Molecular interaction

KW - Polyamine

KW - Z-DNA

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