Integron-associated imipenem resistance in Acinetobacter baumannii isolated from a regional hospital in Taiwan

S. Y. Liu; J. Y. Lin; C. Chu; L. H. Su; T. Y. Lin; C. H. Chiu

doi:10.1016/j.ijantimicag.2005.09.010

Integron-associated imipenem resistance in Acinetobacter baumannii isolated from a regional hospital in Taiwan

S. Y. Liu
, J. Y. Lin
, C. Chu
, L. H. Su
, T. Y. Lin
, C. H. Chiu

Research output: Contribution to journal › Article › peer-review

36 Citations (Scopus)

Abstract

We investigated the genetic properties of imipenem-resistant Acinetobacter baumannii collected from a regional hospital in Taiwan. Pulsed-field gel electrophoresis demonstrated that the isolates were genetically diverse. Polymerase chain reaction, DNA sequencing, and DNA-DNA hybridisation showed that the bla_IMP-1 gene resided as a cassette in a plasmid-borne class 1 integron in two isolates. The majority of the resistant isolates were plasmid-less and carried no bla_IMP, bla_VIM or bla _CFI genes, indicating that other uncharacterised metallo-β- lactamases or mechanisms other than enzyme production are involved in carbapenem resistance in this group of A. baumannii. We conclude that multidrug resistance of A. baumannii was a combined effect of lateral gene transfer and clonal spread of multiple resistant clones. Strict measures should be implemented to control the further spread of resistance.

Original language	English
Pages (from-to)	81-84
Number of pages	4
Journal	International Journal of Antimicrobial Agents
Volume	27
Issue number	1
DOIs	https://doi.org/10.1016/j.ijantimicag.2005.09.010
Publication status	Published - Jan 2006
Externally published	Yes

Keywords

Acinetobacter baumannii
Imipenem resistance
Integron

ASJC Scopus subject areas

Applied Microbiology and Biotechnology
Microbiology
Parasitology
Virology
Immunology and Allergy
Infectious Diseases

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10.1016/j.ijantimicag.2005.09.010

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title = "Integron-associated imipenem resistance in Acinetobacter baumannii isolated from a regional hospital in Taiwan",

abstract = "We investigated the genetic properties of imipenem-resistant Acinetobacter baumannii collected from a regional hospital in Taiwan. Pulsed-field gel electrophoresis demonstrated that the isolates were genetically diverse. Polymerase chain reaction, DNA sequencing, and DNA-DNA hybridisation showed that the blaIMP-1 gene resided as a cassette in a plasmid-borne class 1 integron in two isolates. The majority of the resistant isolates were plasmid-less and carried no blaIMP, blaVIM or bla CFI genes, indicating that other uncharacterised metallo-β- lactamases or mechanisms other than enzyme production are involved in carbapenem resistance in this group of A. baumannii. We conclude that multidrug resistance of A. baumannii was a combined effect of lateral gene transfer and clonal spread of multiple resistant clones. Strict measures should be implemented to control the further spread of resistance.",

keywords = "Acinetobacter baumannii, Imipenem resistance, Integron",

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AU - Liu, S. Y.

AU - Lin, J. Y.

AU - Chu, C.

AU - Su, L. H.

AU - Lin, T. Y.

AU - Chiu, C. H.

PY - 2006/1

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AB - We investigated the genetic properties of imipenem-resistant Acinetobacter baumannii collected from a regional hospital in Taiwan. Pulsed-field gel electrophoresis demonstrated that the isolates were genetically diverse. Polymerase chain reaction, DNA sequencing, and DNA-DNA hybridisation showed that the blaIMP-1 gene resided as a cassette in a plasmid-borne class 1 integron in two isolates. The majority of the resistant isolates were plasmid-less and carried no blaIMP, blaVIM or bla CFI genes, indicating that other uncharacterised metallo-β- lactamases or mechanisms other than enzyme production are involved in carbapenem resistance in this group of A. baumannii. We conclude that multidrug resistance of A. baumannii was a combined effect of lateral gene transfer and clonal spread of multiple resistant clones. Strict measures should be implemented to control the further spread of resistance.

KW - Acinetobacter baumannii

KW - Imipenem resistance

KW - Integron

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Integron-associated imipenem resistance in Acinetobacter baumannii isolated from a regional hospital in Taiwan

Abstract

Keywords

ASJC Scopus subject areas

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