Insulin resistance without obesity induced by cotton pellet granuloma in mice

Hung Tsung Wu, Cheng Kuei Chang, Chiung Wen Tsao, Ya Ju Wen, Sheng Ming Ling, Kai Chun Cheng, Chi Jean Chang, Juei Tang Cheng

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)


Obesity leads to insulin resistance because the larger adipocytes in obese persons secrete proinflammatory cytokines that cause chronic inflammation in adipose tissue. This, in turn, leads to the alteration of adipokine secretion, which can induce insulin resistance. However, the development of insulin resistance without obesity is still obscure. We aimed to use an animal inflammation model with cotton pellet granuloma (CPG) in adipose tissue to characterize insulin resistance formation. We found that CPG in epididymal white adipose tissue (WAT), rather than in interscapular brown adipose tissue, impaired insulin sensitivity, and glucose utilization, and that it decreased levels of phosphoinsulin receptor and phospho-Akt in both muscle and liver tissue, but that it did not modify the body weight or food intake in mice. Macrophage infiltration in adipose tissue, leukocyte counts, monocyte chemoattractant protein-1, and interleukin-6 were elevated in CPG-treated mice. However, we found a marked decrease of plasma adiponectin only in the WAT group, which might have been because of the lower level of peroxisome proliferator-activated receptor-γ in WAT. These results show that granuloma formation in WAT by implantation of a cotton pellet may induce insulin resistance under nonobese condition through circulating inflammatory mediators, especially the low level of adiponectin.

Original languageEnglish
Pages (from-to)362-369
Number of pages8
JournalLaboratory Investigation
Issue number3
Publication statusPublished - Mar 2009


  • Adiponectin
  • Adipose tissue
  • Cotton pellet granuloma
  • Inflammation
  • Insulin resistance

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Molecular Biology
  • Cell Biology


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