Injectable adipose tissue combined with stem cells for soft-tissue augmentation: A pilot study for dental applications

Li Ting Li, Kuang Ta Yao, Shou Cheng Teng, Tiffany P. Sun, Ching Kuo Chen, Chia Chun Chen, Ming Lun Hsu, Hsu Wei Fang

Research output: Contribution to journalArticlepeer-review


Background/purpose Bone resorption and soft-tissue defects are the typical physiologic responses after tooth extraction. Various dental ridge augmentation techniques have been applied and lack of the soft tissue is the major factor causing the failure. We propose that the adipose-derived stem cell can be useful in soft-tissue augmentation in dental applications. The objective of this study was to optimize the operation procedures for the isolation of adipose stem cells and tissues. Accelerated clinical protocols for effective transplantation of adipose tissue with high amount of adipose stem cells shall be developed. Materials and methods Operation parameters were designed and optimized for the extraction of adipose tissue-derived stromal vascular cells. The optimized accelerated procedure was washing the lipoaspirate samples one time. Collagenase was then added and samples were incubated in a water bath for 30 minutes at 37°C and centrifuged at 1200g for 3 minutes. A mouse animal model was applied to evaluate the soft-tissue-filling effects using the optimized procedure. Results The animal model tests demonstrated the filling and regeneration of the soft tissues with significant angiogenesis. Conclusion This pilot study demonstrated the feasibility of soft-tissue augmentation applications.

Original languageEnglish
Pages (from-to)377-386
Number of pages10
JournalJournal of Dental Sciences
Issue number4
Publication statusPublished - Dec 1 2016
Externally publishedYes


  • adipose-derived stem cells
  • alveolar bone resorption
  • cell-assisted lipotransfer
  • ridge defects
  • stromal vascular fraction cells

ASJC Scopus subject areas

  • Dentistry(all)


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