Inhibitory effects of polyphenolic catechins from Chinese green tea on HIV reverse transcriptase activity

C. W. Chang; F. L. Hsu; J. Y. Lin

doi:10.1007/BF02253344

Inhibitory effects of polyphenolic catechins from Chinese green tea on HIV reverse transcriptase activity

C. W. Chang, F. L. Hsu, J. Y. Lin

School of Pharmacy

Research output: Contribution to journal › Article › peer-review

30 Citations (Scopus)

Abstract

Three polyphenolic catechins, epigallocatechin (1), epicatechin-3-O-gallate (2) and epigallocatechin-3-O-gallate (3), were isolated from Chinese green tea, Ti-Kaun-Yin (Camellia sinensis) and demonstrated as a new class of human immunodeficiency virus-reverse transcriptase (HIV-RT) inhibitor. The concentrations required for 50% inhibition for the compounds (1), (2) and (3) were 7.80, 0.32 and 0.68 μM, respectively. The polyphenolic catechins with a galloyl group at the 3 position were potent inhibitors of HIV-RT. Kinetic analysis indicated that the polyphenolic catechins were competitive inhibitors with respect to the template-primer (rA)_n(dT)_12-18 and noncompetitive inhibitors to dTTP.

Original language	English
Pages (from-to)	163-166
Number of pages	4
Journal	Journal of Biomedical Science
Volume	1
Issue number	3
DOIs	https://doi.org/10.1007/BF02253344
Publication status	Published - Jun 1994

Keywords

HIV reverse transcriptase activity
Polyphenolic catechins

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/BF02253344

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title = "Inhibitory effects of polyphenolic catechins from Chinese green tea on HIV reverse transcriptase activity",

abstract = "Three polyphenolic catechins, epigallocatechin (1), epicatechin-3-O-gallate (2) and epigallocatechin-3-O-gallate (3), were isolated from Chinese green tea, Ti-Kaun-Yin (Camellia sinensis) and demonstrated as a new class of human immunodeficiency virus-reverse transcriptase (HIV-RT) inhibitor. The concentrations required for 50% inhibition for the compounds (1), (2) and (3) were 7.80, 0.32 and 0.68 μM, respectively. The polyphenolic catechins with a galloyl group at the 3 position were potent inhibitors of HIV-RT. Kinetic analysis indicated that the polyphenolic catechins were competitive inhibitors with respect to the template-primer (rA)n(dT)12-18 and noncompetitive inhibitors to dTTP.",

keywords = "HIV reverse transcriptase activity, Polyphenolic catechins",

author = "Chang, {C. W.} and Hsu, {F. L.} and Lin, {J. Y.}",

year = "1994",

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doi = "10.1007/BF02253344",

language = "English",

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journal = "Journal of Biomedical Science",

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TY - JOUR

T1 - Inhibitory effects of polyphenolic catechins from Chinese green tea on HIV reverse transcriptase activity

AU - Chang, C. W.

AU - Hsu, F. L.

AU - Lin, J. Y.

PY - 1994/6

Y1 - 1994/6

N2 - Three polyphenolic catechins, epigallocatechin (1), epicatechin-3-O-gallate (2) and epigallocatechin-3-O-gallate (3), were isolated from Chinese green tea, Ti-Kaun-Yin (Camellia sinensis) and demonstrated as a new class of human immunodeficiency virus-reverse transcriptase (HIV-RT) inhibitor. The concentrations required for 50% inhibition for the compounds (1), (2) and (3) were 7.80, 0.32 and 0.68 μM, respectively. The polyphenolic catechins with a galloyl group at the 3 position were potent inhibitors of HIV-RT. Kinetic analysis indicated that the polyphenolic catechins were competitive inhibitors with respect to the template-primer (rA)n(dT)12-18 and noncompetitive inhibitors to dTTP.

AB - Three polyphenolic catechins, epigallocatechin (1), epicatechin-3-O-gallate (2) and epigallocatechin-3-O-gallate (3), were isolated from Chinese green tea, Ti-Kaun-Yin (Camellia sinensis) and demonstrated as a new class of human immunodeficiency virus-reverse transcriptase (HIV-RT) inhibitor. The concentrations required for 50% inhibition for the compounds (1), (2) and (3) were 7.80, 0.32 and 0.68 μM, respectively. The polyphenolic catechins with a galloyl group at the 3 position were potent inhibitors of HIV-RT. Kinetic analysis indicated that the polyphenolic catechins were competitive inhibitors with respect to the template-primer (rA)n(dT)12-18 and noncompetitive inhibitors to dTTP.

KW - HIV reverse transcriptase activity

KW - Polyphenolic catechins

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Inhibitory effects of polyphenolic catechins from Chinese green tea on HIV reverse transcriptase activity

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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