TY - JOUR
T1 - Inhibitory effect of some selected nutraceutic herbs on LDL glycation induced by glucose and glyoxal
AU - Hsieh, Chiu Lan
AU - Lin, Yuh Charn
AU - Ko, Wang Sheng
AU - Peng, Chiung Hui
AU - Huang, Chien Ning
AU - Peng, Robert Y.
N1 - Funding Information:
This research work was supported by the National Science Council, Republic of China (Grant no. 92-2313-B-241-004).
PY - 2005/12/1
Y1 - 2005/12/1
N2 - Anti-LDL glycative agents were investigated using aqueous extracts of Psidium guajava L. (PE), Toona sinensis Roem. (TE), Momordica charantia L. (ME) and Graptopetalum paragugayene E. Walther (GE). Concentrations of extracts 0.01-0.625 mg/mL, low density lipoprotein (LDL; 100 μg protein/mL) and inducers glucose (400 mM) and glyoxal (2.5 mM) were incubated at 37°C. Evaluation parameters involved the thiobarbituric acid reactive substances (TBARS), conjugated dienes (CD), relative electrophoretic mobility (REM), 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging capability and total polyphenolic content. Results for anti-TBARS efficiency (in%) were PE (75.77), TE (75.10), ME (68.81) and GE (19.81) at 0.5 mg/mL, respectively, when induced by glucose; 36.68, 35.60, 32.62 and inactive, respectively, by glyoxal. The lag times for CD formation (in min) were: 289 and 125 by PE and TE, respectively, comparing to the control (45). REM was 1.6 with respect to PE (0.1 mg/mL) compared to the control (4.2). PE at 0.01 mg/mL effectively inhibited with 63.45% efficiency on AGEs induced by glucose. We conclude that PE virtually is a potent antiglycative agent, which can be of great value in the preventive glycation-associated cardiovascular and neurodegenerative diseases.
AB - Anti-LDL glycative agents were investigated using aqueous extracts of Psidium guajava L. (PE), Toona sinensis Roem. (TE), Momordica charantia L. (ME) and Graptopetalum paragugayene E. Walther (GE). Concentrations of extracts 0.01-0.625 mg/mL, low density lipoprotein (LDL; 100 μg protein/mL) and inducers glucose (400 mM) and glyoxal (2.5 mM) were incubated at 37°C. Evaluation parameters involved the thiobarbituric acid reactive substances (TBARS), conjugated dienes (CD), relative electrophoretic mobility (REM), 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging capability and total polyphenolic content. Results for anti-TBARS efficiency (in%) were PE (75.77), TE (75.10), ME (68.81) and GE (19.81) at 0.5 mg/mL, respectively, when induced by glucose; 36.68, 35.60, 32.62 and inactive, respectively, by glyoxal. The lag times for CD formation (in min) were: 289 and 125 by PE and TE, respectively, comparing to the control (45). REM was 1.6 with respect to PE (0.1 mg/mL) compared to the control (4.2). PE at 0.01 mg/mL effectively inhibited with 63.45% efficiency on AGEs induced by glucose. We conclude that PE virtually is a potent antiglycative agent, which can be of great value in the preventive glycation-associated cardiovascular and neurodegenerative diseases.
KW - Glucose
KW - Glycation
KW - Glyoxal
KW - Graptopetalum paragugayene E. Walther
KW - LDL
KW - Momordica charantia L.
KW - Psidium guajava L.
KW - Toona sinensis Roem.
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U2 - 10.1016/j.jep.2005.06.044
DO - 10.1016/j.jep.2005.06.044
M3 - Article
C2 - 16162395
AN - SCOPUS:27644431635
SN - 0378-8741
VL - 102
SP - 357
EP - 363
JO - Journal of Ethnopharmacology
JF - Journal of Ethnopharmacology
IS - 3
ER -