Inhibitory effect of ( - )-epicatechin 4-benzylthioether on the growth of glioma cells in culture

Stephen Shih Fan Hsu; Shuo Bin Jou; Feng Lin Hsu; Tzu Feng Yang; Juei Tang Cheng

doi:10.1002/(SICI)1099-1573(199612)10:8<643::AID-PTR924>3.0.CO;2-1

Inhibitory effect of ( - )-epicatechin 4-benzylthioether on the growth of glioma cells in culture

Stephen Shih Fan Hsu, Shuo Bin Jou, Feng Lin Hsu, Tzu Feng Yang, Juei Tang Cheng

Research output: Contribution to journal › Article › peer-review

Abstract

The effect of (-)-epicatechin 4-benzylthioether (EC-BE) on the growth of cultured rat glioma cells was investigated. EC-BE was found to produce a concentration-dependent inhibitory effect on the growth of glioma cells. Inhibition was also observed in samples treated with (-)-epicatechin acetoxymethyl ester (EC-AM). The original compound (-)-epicatechin, which gave rise to EC-AM and EC-BE, failed to produce a similar result. Because both EC-BE and EC-AM are membrane permeable, while (-)-epicatechin is not, the results indicated that the (-)-epicatechin and its derivatives need to be intracellular in order to produce an effect. Moreover, it is demonstrated that EC-BE inhibited the incorporation of [³H]thymidine into DNA while the uptake of extracellular [³H]thymidine by glioma cells was not affected. The activities of redox enzymes such as glutathione peroxidase and glutathione reductase were attenuated in cells treated with EC-BE. These data suggest that EC-BE can inhibit the growth of glioma cells by affecting a redox pathway related to the formation of DNA.

Original language	English
Pages (from-to)	643-646
Number of pages	4
Journal	Phytotherapy Research
Volume	10
Issue number	8
DOIs	https://doi.org/10.1002/(SICI)1099-1573(199612)10:8<643::AID-PTR924>3.0.CO;2-1
Publication status	Published - Dec 1996
Externally published	Yes

Keywords

(-)-epicatechin
(-)-epicatechin 4-benylthioether
(-)-epicatechin acetoxymethyl ester
Antioxidant
Cell culture
Cell death
Glioma celi

ASJC Scopus subject areas

Pharmacology

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10.1002/(SICI)1099-1573(199612)10:8<643::AID-PTR924>3.0.CO;2-1

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title = "Inhibitory effect of ( - )-epicatechin 4-benzylthioether on the growth of glioma cells in culture",

abstract = "The effect of (-)-epicatechin 4-benzylthioether (EC-BE) on the growth of cultured rat glioma cells was investigated. EC-BE was found to produce a concentration-dependent inhibitory effect on the growth of glioma cells. Inhibition was also observed in samples treated with (-)-epicatechin acetoxymethyl ester (EC-AM). The original compound (-)-epicatechin, which gave rise to EC-AM and EC-BE, failed to produce a similar result. Because both EC-BE and EC-AM are membrane permeable, while (-)-epicatechin is not, the results indicated that the (-)-epicatechin and its derivatives need to be intracellular in order to produce an effect. Moreover, it is demonstrated that EC-BE inhibited the incorporation of [3H]thymidine into DNA while the uptake of extracellular [3H]thymidine by glioma cells was not affected. The activities of redox enzymes such as glutathione peroxidase and glutathione reductase were attenuated in cells treated with EC-BE. These data suggest that EC-BE can inhibit the growth of glioma cells by affecting a redox pathway related to the formation of DNA.",

keywords = "(-)-epicatechin, (-)-epicatechin 4-benylthioether, (-)-epicatechin acetoxymethyl ester, Antioxidant, Cell culture, Cell death, Glioma celi",

author = "Hsu, {Stephen Shih Fan} and Jou, {Shuo Bin} and Hsu, {Feng Lin} and Yang, {Tzu Feng} and Cheng, {Juei Tang}",

year = "1996",

month = dec,

doi = "10.1002/(SICI)1099-1573(199612)10:8<643::AID-PTR924>3.0.CO;2-1",

language = "English",

volume = "10",

pages = "643--646",

journal = "Phytotherapy Research",

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T1 - Inhibitory effect of ( - )-epicatechin 4-benzylthioether on the growth of glioma cells in culture

AU - Hsu, Stephen Shih Fan

AU - Jou, Shuo Bin

AU - Hsu, Feng Lin

AU - Yang, Tzu Feng

AU - Cheng, Juei Tang

PY - 1996/12

Y1 - 1996/12

N2 - The effect of (-)-epicatechin 4-benzylthioether (EC-BE) on the growth of cultured rat glioma cells was investigated. EC-BE was found to produce a concentration-dependent inhibitory effect on the growth of glioma cells. Inhibition was also observed in samples treated with (-)-epicatechin acetoxymethyl ester (EC-AM). The original compound (-)-epicatechin, which gave rise to EC-AM and EC-BE, failed to produce a similar result. Because both EC-BE and EC-AM are membrane permeable, while (-)-epicatechin is not, the results indicated that the (-)-epicatechin and its derivatives need to be intracellular in order to produce an effect. Moreover, it is demonstrated that EC-BE inhibited the incorporation of [3H]thymidine into DNA while the uptake of extracellular [3H]thymidine by glioma cells was not affected. The activities of redox enzymes such as glutathione peroxidase and glutathione reductase were attenuated in cells treated with EC-BE. These data suggest that EC-BE can inhibit the growth of glioma cells by affecting a redox pathway related to the formation of DNA.

AB - The effect of (-)-epicatechin 4-benzylthioether (EC-BE) on the growth of cultured rat glioma cells was investigated. EC-BE was found to produce a concentration-dependent inhibitory effect on the growth of glioma cells. Inhibition was also observed in samples treated with (-)-epicatechin acetoxymethyl ester (EC-AM). The original compound (-)-epicatechin, which gave rise to EC-AM and EC-BE, failed to produce a similar result. Because both EC-BE and EC-AM are membrane permeable, while (-)-epicatechin is not, the results indicated that the (-)-epicatechin and its derivatives need to be intracellular in order to produce an effect. Moreover, it is demonstrated that EC-BE inhibited the incorporation of [3H]thymidine into DNA while the uptake of extracellular [3H]thymidine by glioma cells was not affected. The activities of redox enzymes such as glutathione peroxidase and glutathione reductase were attenuated in cells treated with EC-BE. These data suggest that EC-BE can inhibit the growth of glioma cells by affecting a redox pathway related to the formation of DNA.

KW - (-)-epicatechin

KW - (-)-epicatechin 4-benylthioether

KW - (-)-epicatechin acetoxymethyl ester

KW - Antioxidant

KW - Cell culture

KW - Cell death

KW - Glioma celi

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Inhibitory effect of ( - )-epicatechin 4-benzylthioether on the growth of glioma cells in culture

Abstract

Keywords

ASJC Scopus subject areas

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