TY - JOUR
T1 - Inhibition of Epstein-Barr virus lytic cycle by (-)-epigallocatechin gallate
AU - Chang, Li Kwan
AU - Wei, Ta Tung
AU - Chiu, Ya Fang
AU - Tung, Chao Ping
AU - Chuang, Jian Ying
AU - Hung, Shang Kai
AU - Li, Ching
AU - Liu, Shih Tung
N1 - Funding Information:
The authors thank Professor M. Chao for providing anti-EBNA-1 antibody. Kuan-Yin Shen is also appreciated for assisting in the flow cytometry analysis. This work was supported by a Medical Research Grant, CMRP720-VI, from Chang-Gung Memorial Hospital, a research grant from the National Health Research Institute of the ROC (NHRI-EX91-8901SL), and grants (NSC91-2320-B-037-014 and NSC91-2320-B-182-048) from the National Science Council of the ROC, and a research fund (CSMU 90-OM-B-018) from Chung-Shan Medical University.
PY - 2003/2/21
Y1 - 2003/2/21
N2 - (-)-Epigallocatechin gallate (EGCG), abundant in green tea, is a potent anti-microbial and anti-tumor compound. This investigation used immunoblot, flow cytometry, microarray, and indirect immunofluorescence analyses to show that at concentrations exceeding 50μM, EGCG inhibits the expression of Epstein-Barr virus (EBV) lytic proteins, including Rta, Zta, and EA-D, but does not affect the expression of EBNA-1. Moreover, DNA microarray and transient transfection analyses demonstrated that EGCG blocks EBV lytic cycle by inhibiting the transcription of immediate-early genes, thus inhibiting the initiation of EBV lytic cascade.
AB - (-)-Epigallocatechin gallate (EGCG), abundant in green tea, is a potent anti-microbial and anti-tumor compound. This investigation used immunoblot, flow cytometry, microarray, and indirect immunofluorescence analyses to show that at concentrations exceeding 50μM, EGCG inhibits the expression of Epstein-Barr virus (EBV) lytic proteins, including Rta, Zta, and EA-D, but does not affect the expression of EBNA-1. Moreover, DNA microarray and transient transfection analyses demonstrated that EGCG blocks EBV lytic cycle by inhibiting the transcription of immediate-early genes, thus inhibiting the initiation of EBV lytic cascade.
UR - https://www.scopus.com/pages/publications/0037459049
UR - https://www.scopus.com/pages/publications/0037459049#tab=citedBy
U2 - 10.1016/S0006-291X(03)00067-6
DO - 10.1016/S0006-291X(03)00067-6
M3 - Article
C2 - 12589821
AN - SCOPUS:0037459049
SN - 0006-291X
VL - 301
SP - 1062
EP - 1068
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 4
ER -