Integrins regulate cell growth, differentiation, and behavior in many systems. Integrin β1C (β1S) is an alternatively spliced variant of integrin β1 with a specific cytoplasmic domain and is expressed in several human tissues. Human β1C transiently expressed in mouse 10T1/2 fibroblasts showed a diffuse pattern of cell surface staining, whereas β1 localized to focal adhesions. Moderate concentrations of β1C had no effect on actin stress fibers or focal adhesions, but markedly inhibited DNA synthesis. Inhibition by β1C mapped to the late G1 phase of the cell cycle, near the G1-S boundary. Thus, alternative splicing of β1 results in transmission of distinct signals that may regulate growth in vivo.
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