Influenza virus a stimulates expression of eotaxin by nasal epithelial cells

M. Kawaguchi, F. Kokubu, H. Kuga, T. Tomita, S. Matsukura, H. Suzaki, S. K. Huang, M. Adachi

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14 Citations (Scopus)


Background: Respiratory virus is one of the most common causes of airway inflammation, but its pathogenic mechanisms are not well understood. Eotaxin is a potent eosinophil chemoattractant and is a selective agonist for C-C chemokine receptor 3 (CCR3). Although it has recently been demonstrated that epithelial cells express eotaxin, both in vivo and in vitro, there are few data concerning the expression in viral infection. Objects: We hypothesized that eotaxin may play an important role in attracting inflammatory cells into the airway after viral infection and analysed whether viral infection induces eotaxin in nasal epithelial cells in vitro. Methods: Nasal epithelial cells obtained from polypectomy for nasal polyp were infected with influenza virus A (subtype H3N2). The cells and supernatants were collected 8, 24 and 48 h after infection. Eotaxin mRNA was analysed by RT-PCR. Eotaxin concentration in the supernatants was analysed by enzyme-linked immunosorbent assay. We also examined a blocking assay to analyse the intervention of pro-inflammatory cytokines, TNF-α and IL-β in eotaxin production induced by influenza virus. Results: The results showed that eotaxin was expressed constitutively in uninfected cells, but was up-regulated for both mRNA and protein levels in infected cells. Blocking experiments using anti-TNF-α and anti-IL-β antibodies showed no effects of these agents on the level of eotaxin. In addition, UV-inactivated virus did not enhance the expression of eotaxin. Conclusions: These results suggest that influenza virus A infection in nasal epithelial cells stimulates the expression of eotaxin, and may play an important role in the pathogenesis of airway inflammation by inducing eotaxin.

Original languageEnglish
Pages (from-to)873-880
Number of pages8
JournalClinical and Experimental Allergy
Issue number6
Publication statusPublished - 2001


  • Airway inflammation
  • Eotaxin
  • Influenza virus
  • Nasal epithelial cells

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology


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