TY - JOUR
T1 - Influence of serotonergic mechanisms on the urine flow rate in male rats
AU - Fan, Wen Jia
AU - Chen, Shih-Ching
AU - Hsieh, Tsung-hsun
AU - Lai, Chien-Hung
AU - Lin, You-Shuei
AU - Peng, Chih-Wei
AU - Kou, Yu Ru
N1 - Publisher Copyright:
© 2014 the American Physiological Society.
PY - 2014/11/15
Y1 - 2014/11/15
N2 - This study extensively examined the role of a 5-HT1A receptor in controlling voiding function in anesthetized male rats. A simultaneous recording of the intravesical pressure (IVP), external urethral sphincter (EUS)-electromyography (EMG), and urine flow rate (UFR) during continuous cystometry was used. 8-Hydroxy-2-(di-n-propylamino)-tetralin (8-OH-DPAT), a 5-HT1A receptor agonist, significantly improved the voiding efficiency, as detected by increases in the evoked contraction amplitude, EUS burst period, and silent period, and decreases in the volume threshold, pressure threshold, and residual volume. Interestingly, the UFR during voiding was reduced by 8-OH-DPAT, as evidenced by decreases in the maximal UFR and mean UFRs of the voiding period, spike duration, and interspike interval. Conversely, treating rats with WAY-100635, a 5-HT1A antagonist, produced effects opposite to those produced by 8-OH-DPAT. These findings suggest that 8-OH-DPAT improved the voiding efficiency by enhancing the detrusor contractile ability and prolonging EUS burst period, which would compensate for the lower UFR, resulting from urethral smooth muscle contractions and longer EUS silent periods during voiding. The present study contributes to our understanding of the role of 5-HT1A receptors in controlling the urine flow rate in male rats.
AB - This study extensively examined the role of a 5-HT1A receptor in controlling voiding function in anesthetized male rats. A simultaneous recording of the intravesical pressure (IVP), external urethral sphincter (EUS)-electromyography (EMG), and urine flow rate (UFR) during continuous cystometry was used. 8-Hydroxy-2-(di-n-propylamino)-tetralin (8-OH-DPAT), a 5-HT1A receptor agonist, significantly improved the voiding efficiency, as detected by increases in the evoked contraction amplitude, EUS burst period, and silent period, and decreases in the volume threshold, pressure threshold, and residual volume. Interestingly, the UFR during voiding was reduced by 8-OH-DPAT, as evidenced by decreases in the maximal UFR and mean UFRs of the voiding period, spike duration, and interspike interval. Conversely, treating rats with WAY-100635, a 5-HT1A antagonist, produced effects opposite to those produced by 8-OH-DPAT. These findings suggest that 8-OH-DPAT improved the voiding efficiency by enhancing the detrusor contractile ability and prolonging EUS burst period, which would compensate for the lower UFR, resulting from urethral smooth muscle contractions and longer EUS silent periods during voiding. The present study contributes to our understanding of the role of 5-HT1A receptors in controlling the urine flow rate in male rats.
KW - 8-hydroxy-2-(di-n-propylamino)-tetralin
KW - External urethral sphincter-electromyography
KW - Intravesical pressure
KW - Voiding efficiency
KW - WAY-100635
UR - http://www.scopus.com/inward/record.url?scp=84922393183&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84922393183&partnerID=8YFLogxK
U2 - 10.1152/ajpregu.00160.2014
DO - 10.1152/ajpregu.00160.2014
M3 - Article
C2 - 25209414
AN - SCOPUS:84922393183
SN - 0363-6119
VL - 307
SP - R1239-R1250
JO - American Journal of Physiology - Regulatory Integrative and Comparative Physiology
JF - American Journal of Physiology - Regulatory Integrative and Comparative Physiology
IS - 10
ER -