Induction of insulin-like growth factors expression in dog mandibles by β-TCP

Kung Liang Chao; Ujjal K. Bhawal; Takao Watanabe; Tsuneo Takahashi; Wei Jen Chang; Sheng Yang Lee; Yoshimitsu Abiko

doi:10.2485/jhtb.21.1

Induction of insulin-like growth factors expression in dog mandibles by β-TCP

Kung Liang Chao, Ujjal K. Bhawal, Takao Watanabe, Tsuneo Takahashi, Wei Jen Chang, Sheng Yang Lee, Yoshimitsu Abiko

Research output: Contribution to journal › Article › peer-review

Abstract

To understand the usefulness of β-TCP for development of biomaterials implants, β-TCP was implanted into bone defects of dog mandibles, and gene expression profiles were examined using DNA microarray. An implant drill was used to make bone defects in Beagle dog mandibles, and then β-TCP was filled into bone defects. Total RNA was isolated from all specimens, and mRNA levels were analyzed using Affymetrix GeneChip. Higher mRNA levels of insulin-like growth factors, IGF1 and IGF2, were observed in β-TCP-implanted samples compared with controls. The enhancement of IGF1 and IGF2 mRNA levels by β-TCP was confirmed by RT-PCR and real-time RT-PCR. Immunohistochemical staining revealed increased IGF1 and IGF2 protein expression in β-TCP-implanted bone. Taken together, the stimulation of IGF1 and IGF2 expression by β-TCP might be a mechanism of accelerating bone formation.

Original language	English
Pages (from-to)	1-8
Number of pages	8
Journal	Journal of Hard Tissue Biology
Volume	21
Issue number	1
DOIs	https://doi.org/10.2485/jhtb.21.1
Publication status	Published - 2012

Keywords

Dog mandible
Gene expression
Insulin-like growth factor (IGF)
β- TCP

ASJC Scopus subject areas

General Dentistry
Biochemistry
Medicine (miscellaneous)
Cell Biology
Biomaterials
Orthopedics and Sports Medicine

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10.2485/jhtb.21.1

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title = "Induction of insulin-like growth factors expression in dog mandibles by β-TCP",

abstract = "To understand the usefulness of β-TCP for development of biomaterials implants, β-TCP was implanted into bone defects of dog mandibles, and gene expression profiles were examined using DNA microarray. An implant drill was used to make bone defects in Beagle dog mandibles, and then β-TCP was filled into bone defects. Total RNA was isolated from all specimens, and mRNA levels were analyzed using Affymetrix GeneChip. Higher mRNA levels of insulin-like growth factors, IGF1 and IGF2, were observed in β-TCP-implanted samples compared with controls. The enhancement of IGF1 and IGF2 mRNA levels by β-TCP was confirmed by RT-PCR and real-time RT-PCR. Immunohistochemical staining revealed increased IGF1 and IGF2 protein expression in β-TCP-implanted bone. Taken together, the stimulation of IGF1 and IGF2 expression by β-TCP might be a mechanism of accelerating bone formation.",

keywords = "Dog mandible, Gene expression, Insulin-like growth factor (IGF), β- TCP",

author = "Chao, {Kung Liang} and Bhawal, {Ujjal K.} and Takao Watanabe and Tsuneo Takahashi and Chang, {Wei Jen} and Lee, {Sheng Yang} and Yoshimitsu Abiko",

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doi = "10.2485/jhtb.21.1",

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AU - Chao, Kung Liang

AU - Bhawal, Ujjal K.

AU - Watanabe, Takao

AU - Takahashi, Tsuneo

AU - Chang, Wei Jen

AU - Lee, Sheng Yang

AU - Abiko, Yoshimitsu

PY - 2012

Y1 - 2012

N2 - To understand the usefulness of β-TCP for development of biomaterials implants, β-TCP was implanted into bone defects of dog mandibles, and gene expression profiles were examined using DNA microarray. An implant drill was used to make bone defects in Beagle dog mandibles, and then β-TCP was filled into bone defects. Total RNA was isolated from all specimens, and mRNA levels were analyzed using Affymetrix GeneChip. Higher mRNA levels of insulin-like growth factors, IGF1 and IGF2, were observed in β-TCP-implanted samples compared with controls. The enhancement of IGF1 and IGF2 mRNA levels by β-TCP was confirmed by RT-PCR and real-time RT-PCR. Immunohistochemical staining revealed increased IGF1 and IGF2 protein expression in β-TCP-implanted bone. Taken together, the stimulation of IGF1 and IGF2 expression by β-TCP might be a mechanism of accelerating bone formation.

AB - To understand the usefulness of β-TCP for development of biomaterials implants, β-TCP was implanted into bone defects of dog mandibles, and gene expression profiles were examined using DNA microarray. An implant drill was used to make bone defects in Beagle dog mandibles, and then β-TCP was filled into bone defects. Total RNA was isolated from all specimens, and mRNA levels were analyzed using Affymetrix GeneChip. Higher mRNA levels of insulin-like growth factors, IGF1 and IGF2, were observed in β-TCP-implanted samples compared with controls. The enhancement of IGF1 and IGF2 mRNA levels by β-TCP was confirmed by RT-PCR and real-time RT-PCR. Immunohistochemical staining revealed increased IGF1 and IGF2 protein expression in β-TCP-implanted bone. Taken together, the stimulation of IGF1 and IGF2 expression by β-TCP might be a mechanism of accelerating bone formation.

KW - Dog mandible

KW - Gene expression

KW - Insulin-like growth factor (IGF)

KW - β- TCP

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Induction of insulin-like growth factors expression in dog mandibles by β-TCP

Abstract

Keywords

ASJC Scopus subject areas

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