TY - JOUR
T1 - Induction of fatty acid cyclooxygenase in rat aortic smooth muscle cells by estradiol
AU - Chang, Wen Chang
AU - Nakao, Junko
AU - Murota, Sei itsu
AU - Tai, Hsin Hsiung
N1 - Funding Information:
This work was supported in part from the National Institutes of Health (GM-30380), the American Heart Association (81-1152), the Kentucky Heart Association and the University of Kentucky Isabell L, Kircher Fund.
PY - 1983/1
Y1 - 1983/1
N2 - We have previously reported that estradiol stimulates prostacyclin biosynthesis in cultured rat aortic smooth muscle cells by increasing fatty acid cyclooxygenase activity, and induction of new protein biosynthesis is involved since the effect of estradiol is blocked by cycloheximide treatment. In order to see whether estradiol stimulates de novo biosynthesis of fatty acid cyclooxygenase in cells, a specific radioimmunoassay of fatty acid cyclooxygenase was used to measure the enzyme. Estradiol significantly increased immunoreactive fatty acid cyclooxygenase in cells. Consequently, increase in cyclooxygenase activity results in the production of more prostaglandin endoperoxides for final increased synthesis of prostacyclin in these cells.
AB - We have previously reported that estradiol stimulates prostacyclin biosynthesis in cultured rat aortic smooth muscle cells by increasing fatty acid cyclooxygenase activity, and induction of new protein biosynthesis is involved since the effect of estradiol is blocked by cycloheximide treatment. In order to see whether estradiol stimulates de novo biosynthesis of fatty acid cyclooxygenase in cells, a specific radioimmunoassay of fatty acid cyclooxygenase was used to measure the enzyme. Estradiol significantly increased immunoreactive fatty acid cyclooxygenase in cells. Consequently, increase in cyclooxygenase activity results in the production of more prostaglandin endoperoxides for final increased synthesis of prostacyclin in these cells.
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U2 - 10.1016/S0262-1746(83)80018-3
DO - 10.1016/S0262-1746(83)80018-3
M3 - Article
C2 - 6402792
AN - SCOPUS:0020693279
SN - 0262-1746
VL - 10
SP - 33
EP - 37
JO - Prostaglandins, Leukotrienes and Medicine
JF - Prostaglandins, Leukotrienes and Medicine
IS - 1
ER -