TY - JOUR
T1 - Increasing expression of extracellular matrix metalloprotease inducer in renal cell carcinoma
T2 - Tissue microarray analysis of immunostaining score with clinicopathological parameters
AU - Jin, Jong Shiaw
AU - Hsieh, Dar Shih
AU - Lin, Yeh Feng
AU - Wang, Jia Yi
AU - Sheu, Lai Fa
AU - Lee, Wei Hwa
PY - 2006/5
Y1 - 2006/5
N2 - Aim: Renal tumor cell invasion is responsible for both local tissue destruction and distant metastasis. Invasion is largely mediated by matrix metalloproteases that are thought to be induced by tumor cell-derived extracellular matrix metalloprotease inducer (EMMPRIN) in surrounding fibroblasts. We hypothesized that EMMPRIN and matrix metalloproteinase-9 (MMP-9) are over-expressed in renal cell carcinoma. Methods: Immunohistochemical analysis of EMMPRIN and MMP-9 was performed in tissue microarrays of 79 renal cell carcinomas including 12 cases of chromophobe renal cell carcinoma (ChRCC), 53 cases of clear cell renal cell carcinoma (CRCC), 8 cases of papillary renal cell carcinoma (PRCC), and 6 cases of carcinoma of the collecting ducts of Bellini (CoRCC). Results: All renal cell carcinomas showed significant immunohistochemical expression of EMMPRIN. The EMMPRIN score in ChRCC (321 ± 21) was significantly higher than in other histological subtypes of RCC (166 ± 19 for CRCC; 276 ± 24 for PRCC; 98 ± 17 for CoRCC). MMP-9 was mainly expressed in tumor stromal cells and not in non-cancerous fibrovascular regions. The percent positive staining of MMP-9 at the invasive front of tumor cells was significantly higher in CRCC than in ChRCC, PRCC, or CoRCC. Higher EMMPRIN scores in CRCC were associated with shorter survival time, and correlated with higher T staging and nuclear grading. Conclusions: Our findings demonstrate for the first time that EMMPRIN is over-expressed in renal cell carcinomas. Increased expression of EMMPRIN in tumor cells is associated with poor prognosis of patients with CRCC.
AB - Aim: Renal tumor cell invasion is responsible for both local tissue destruction and distant metastasis. Invasion is largely mediated by matrix metalloproteases that are thought to be induced by tumor cell-derived extracellular matrix metalloprotease inducer (EMMPRIN) in surrounding fibroblasts. We hypothesized that EMMPRIN and matrix metalloproteinase-9 (MMP-9) are over-expressed in renal cell carcinoma. Methods: Immunohistochemical analysis of EMMPRIN and MMP-9 was performed in tissue microarrays of 79 renal cell carcinomas including 12 cases of chromophobe renal cell carcinoma (ChRCC), 53 cases of clear cell renal cell carcinoma (CRCC), 8 cases of papillary renal cell carcinoma (PRCC), and 6 cases of carcinoma of the collecting ducts of Bellini (CoRCC). Results: All renal cell carcinomas showed significant immunohistochemical expression of EMMPRIN. The EMMPRIN score in ChRCC (321 ± 21) was significantly higher than in other histological subtypes of RCC (166 ± 19 for CRCC; 276 ± 24 for PRCC; 98 ± 17 for CoRCC). MMP-9 was mainly expressed in tumor stromal cells and not in non-cancerous fibrovascular regions. The percent positive staining of MMP-9 at the invasive front of tumor cells was significantly higher in CRCC than in ChRCC, PRCC, or CoRCC. Higher EMMPRIN scores in CRCC were associated with shorter survival time, and correlated with higher T staging and nuclear grading. Conclusions: Our findings demonstrate for the first time that EMMPRIN is over-expressed in renal cell carcinomas. Increased expression of EMMPRIN in tumor cells is associated with poor prognosis of patients with CRCC.
KW - Clear cell subtype
KW - EMMPRIN
KW - Extracellular matrix metalloprotease inducer
KW - Matrix metalloprotease-9
KW - Renal cell carcinoma
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U2 - 10.1111/j.1442-2042.2006.01353.x
DO - 10.1111/j.1442-2042.2006.01353.x
M3 - Article
C2 - 16771728
AN - SCOPUS:33646810120
SN - 0919-8172
VL - 13
SP - 573
EP - 580
JO - International Journal of Urology
JF - International Journal of Urology
IS - 5
ER -